Juvenile hormone diol kinase. II. Sequencing, cloning, and molecular modeling of juvenile hormone-selective diol kinase from Manduca sexta

The Journal of Biological Chemistry
Robert A MaxwellD A Schooley

Abstract

The gene sequence of Manduca sexta juvenile hormone diol kinase (JHDK) codes for an enzyme that has 59% sequence identity to Drosophila melanogaster sarcoplasmic calcium-binding protein-2 (dSCP2). JHDK and dSCP2 are similar to G-proteins with three conserved sequence elements involved in purine nucleotide binding. Both proteins contain two pairs of EF-hand motifs. Characterization and partial purification of the D. melanogaster homolog of M. sexta JHDK from adult D. melanogaster gave material with JHDK activity. This activity has an experimental pI and molecular mass that are nearly identical to those of dSCP2. Moreover, D. melanogaster phosphotransferase activity has very similar chromatographic retention in three systems compared with M. sexta JHDK. Substrate docking to three-dimensional models of JHDK has shown that the three conserved nucleotide-binding elements surround the putative substrate-binding site and align with conserved sequence elements of p21(Ras) and adenylate kinase. D. melanogaster dSCP2 is a homolog of M. sexta JHDK, and these proteins constitute a novel kinase family that binds nucleotides using the scaffold of an SCP (Protein Data Bank code ).

References

Jun 25, 1991·Nucleic Acids Research·D R Cavener, S C Ray
Oct 5, 1991·Journal of Molecular Biology·E J Milner-WhiteI A Anton
Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T E DeverW C Merrick
Sep 10, 1970·Biochemical and Biophysical Research Communications·D PiszkiewiczE L Smith
Jan 1, 1983·Methods in Enzymology·A S Inglis
Feb 23, 1996·Journal of Molecular Biology·G J SchlaudererG E Schulz
Aug 2, 1996·Science·L Holm, C Sander
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·T J NelsonD L Alkon
Feb 1, 1997·Journal of Structural Biology·O LlorcaJ M Valpuesta
Mar 28, 1997·The Journal of Biological Chemistry·A MatteL T Delbaere
Jan 1, 1997·Annual Review of Biochemistry·S R Sprang
Dec 5, 1998·Journal of Molecular Biology·M de RinaldisM Helmer-Citterich
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·T I ZarembinskiS H Kim
Jan 14, 1999·Nature Structural Biology·S RétyA Lewit-Bentley
Jun 11, 1999·Current Opinion in Structural Biology·C A OrengoJ M Thornton
Oct 16, 1999·Nature·J W Truman, L M Riddiford
Dec 11, 1999·Nucleic Acids Research·L Lo ConteC Chothia
May 9, 2000·Quarterly Reviews of Biophysics·I R Vetter, A Wittinghofer
Dec 15, 2000·Current Opinion in Structural Biology·A Lewit-Bentley, S Réty
Apr 18, 2001·The Journal of Biological Chemistry·Z GombosM Ikura

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Citations

Aug 31, 2010·Journal of Pesticide Science·Shizuo G Kamita, Bruce D Hammock
Jan 16, 2007·Biochimica Et Biophysica Acta·Michael R YeamanWilliam H Welch
Jun 30, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yongping GaoMichele G Wheatly
Mar 21, 2006·Biochemical and Biophysical Research Communications·Zoltan GombosAvijit Chakrabartty
Oct 6, 2005·Insect Biochemistry and Molecular Biology·Sheng LiDavid A Schooley
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·P T ErskineJ B Cooper
Apr 13, 2017·Scientific Reports·Abudourusuli TusunGuirong Wang
Nov 30, 2020·International Journal of Biological Macromolecules·Haiyang XuPing Zhao
Feb 11, 2009·Journal of Proteome Research·Liudy GarciaMarcelo Valle de Sousa
Feb 1, 2005·Insect Biochemistry and Molecular Biology·Qi-Rui ZhangSheng Li
Dec 7, 2021·Acta Crystallographica. Section F, Structural Biology Communications·Jingxu GuoJonathan B Cooper

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