PMID: 11607060Jan 1, 1990Paper

Juvenile hormone receptors in insect larval epidermis: identification by photoaffinity labeling

Proceedings of the National Academy of Sciences of the United States of America
S R PalliL M Riddiford

Abstract

Tritiated photoaffinity analogs of the natural lepidopteran juvenile hormones, JH I and II [epoxy[3H]bishomofarnesyl diazoacetate ([3H]EBDA) and epoxy[3H]homofarnesyl diazoacetate ([3H]EHDA)], and of the JH analog methoprene [[3H]methoprene diazoketone ([3H]MDK)] were synthesized and used to identify specific JH binding proteins in the larval epidermis of the tobacco hornworm (Manduca sexta). EBDA and EHDA specifically photolabeled a 29-kDa nuclear protein (pI 5.8). This protein and a second 29-kDa protein (pI 6.0) were labeled by MDK, but excess unlabeled methoprene or MDK only prevented binding to the latter. These 29-kDa proteins are also present in larval fat body but not in epidermis from either wandering stage or allatectomized larvae, which lack high-affinity JH binding sites. A 29-kDa nuclear protein with the same developmental specificity as this JH binder bound the DNA of two larval endocuticle genes. A 38-kDa cytosolic protein was also specifically photolabeled by these photoaffinity analogs. The 29-kDa nuclear protein is likely the high-affinity receptor for JH that mediates its genomic action, whereas the 38-kDa cytosolic protein may serve as an intracellular carrier for these highly lipophilic hormones and hormone...Continue Reading

References

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Sep 20, 1988·Journal of Molecular Biology·J E Rebers, L M Riddiford
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G D Prestwich, C Wawrzeńczyk
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Citations

Jan 1, 1996·Archives of Insect Biochemistry and Physiology·L M Riddiford
Sep 1, 1994·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K KurataH Shinbo
Sep 1, 1994·Insect Biochemistry and Molecular Biology·G D PrestwichB D Hammock
Sep 1, 1995·Insect Biochemistry and Molecular Biology·V C Henrich, N E Brown
Jul 16, 1999·Insect Biochemistry and Molecular Biology·J A TillmanG J Blomquist
May 13, 2009·Molecular and Cellular Endocrinology·Jun WangWalter G Goodman
Jan 31, 1992·Biochemical and Biophysical Research Communications·K Touhara, G D Prestwich
Nov 1, 1990·General and Comparative Endocrinology·G D PrestwichE S Chang
Aug 6, 2000·Developmental Biology·E B DubrovskyE M Berger
Jan 10, 2006·Vitamins and Hormones·Edward M Berger, Edward B Dubrovsky
Oct 11, 2019·Annual Review of Entomology·Lynn M Riddiford
Sep 1, 1995·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Klaus HartfelderChristine Hepperle
Mar 2, 1993·Biochemistry·K TouharaG D Prestwich

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