Juvenile porcine islets can restore euglycemia in diabetic athymic nude mice after xenotransplantation

Transplantation
Rahul KrishnanJ R Lakey

Abstract

Porcine islet xenotransplantation has been demonstrated in many animal studies to cure experimentally induced diabetes. However, several issues currently impede the translation of porcine islet xenotransplantation to sustained insulin independence clinically. Although adult pigs have mature islets that secrete insulin in response to a glucose challenge, and are physiologically similar to humans, there are logistical considerations with adult porcine tissue that are not present with juvenile porcine tissue. To circumvent these issues, we have identified 18- to 21-day-old preweaned juvenile pigs as islet donors as we have previously demonstrated superior islet yields and function from juvenile pigs using our islet isolation protocols. We evaluated the efficacy of islets isolated from 18- to 24-day-old Yorkshire swine in vitro using a standard glucose-stimulated insulin response assay, and in vivo after xenotransplantation under the kidney capsule of streptozotocin-induced 8- to 10-week-old male athymic nude mice. The mice were monitored for a period of 60 days after transplantation, after which the grafts were explanted and analyzed. Diabetic athymic nude mice transplanted with 1500 to 3000 islet equivalents (IEq) of islets achie...Continue Reading

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Citations

Oct 4, 2015·Biochemical Pharmacology·Aileen King, James Bowe
Mar 9, 2017·Current Diabetes Reports·Bassem F Salama, Gregory S Korbutt
Mar 25, 2016·World Journal of Transplantation·Uriel BarkaiPaul de Vos
Jun 17, 2020·American Journal of Physiology. Endocrinology and Metabolism·Clarissa Hernandez StephensSherry Voytik-Harbin

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