K+-Cl- Cotransporter-3a Up-regulates Na+,K+-ATPase in Lipid Rafts of Gastric Luminal Parietal Cells

The Journal of Biological Chemistry
Takuto FujiiHideki Sakai

Abstract

Gastric parietal cells migrate from the luminal to the basal region of the gland, and they gradually lose acid secretory activity. So far, distribution and function of K+-Cl(-) cotransporters (KCCs) in gastric parietal cells have not been reported. We found that KCC3a but not KCC3b mRNA was highly expressed, and KCC3a protein was predominantly expressed in the basolateral membrane of rat gastric parietal cells located in the luminal region of the glands. KCC3a and the Na+,K+-ATPase alpha1-subunit (alpha1NaK) were coimmunoprecipitated, and both of them were highly localized in a lipid raft fraction. The ouabain-sensitive K+-dependent ATP-hydrolyzing activity (Na+,K+-ATPase activity) was significantly inhibited by a KCC inhibitor (R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]acetic acid (DIOA)). The stable exogenous expression of KCC3a in LLC-PK1 cells resulted in association of KCC3a with endogenous alpha1NaK, and it recruited alpha1NaK in lipid rafts, accompanying increases of Na+,K+-ATPase activity and ouabain-sensitive Na+ transport activity that were suppressed by DIOA, whereas the total expression level of alpha1NaK in the cells was not significantly altered. On the other hand, the expres...Continue Reading

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Citations

Sep 21, 2011·The Journal of Physiological Sciences : JPS·Takuto FujiiHideki Sakai
Aug 6, 2009·Current Opinion in Nephrology and Hypertension·Xiaoyan WangPedro A Jose
Aug 5, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Peter M PiermariniKlaus W Beyenbach
Apr 15, 2011·Journal of Lipids·Katie D Hickey, Mary M Buhr
Dec 14, 2011·BMC Research Notes·Anna-Maria Hartmann, Hans Gerd Nothwang
Jun 2, 2011·Biological & Pharmaceutical Bulletin·Takuto FujiiHideki Sakai
Aug 11, 2011·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Takuto FujiiHideki Sakai
Jun 27, 2012·Biochemical and Biophysical Research Communications·Kyosuke FujitaHideki Sakai
Aug 10, 2010·Biochemical and Biophysical Research Communications·Takuto FujiiHideki Sakai
Aug 21, 2015·Chemistry and Physics of Lipids·Elisabeth A CalhoonJoseph B Williams
Aug 19, 2009·Journal of Neurochemistry·Anna-Maria HartmannHans Gerd Nothwang
Nov 17, 2009·Biology of the Cell·Monica CarmosinoMaria Svelto
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Oct 4, 2013·American Journal of Physiology. Renal Physiology·Zesergio MeloGerardo Gamba
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Aug 18, 2017·American Journal of Physiology. Cell Physiology·A P GarneauP Isenring
Oct 19, 2020·Cellular and Molecular Gastroenterology and Hepatology·Tyler J CaronSusan J Hagen

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