Abstract
Gastric parietal cells migrate from the luminal to the basal region of the gland, and they gradually lose acid secretory activity. So far, distribution and function of K+-Cl(-) cotransporters (KCCs) in gastric parietal cells have not been reported. We found that KCC3a but not KCC3b mRNA was highly expressed, and KCC3a protein was predominantly expressed in the basolateral membrane of rat gastric parietal cells located in the luminal region of the glands. KCC3a and the Na+,K+-ATPase alpha1-subunit (alpha1NaK) were coimmunoprecipitated, and both of them were highly localized in a lipid raft fraction. The ouabain-sensitive K+-dependent ATP-hydrolyzing activity (Na+,K+-ATPase activity) was significantly inhibited by a KCC inhibitor (R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]acetic acid (DIOA)). The stable exogenous expression of KCC3a in LLC-PK1 cells resulted in association of KCC3a with endogenous alpha1NaK, and it recruited alpha1NaK in lipid rafts, accompanying increases of Na+,K+-ATPase activity and ouabain-sensitive Na+ transport activity that were suppressed by DIOA, whereas the total expression level of alpha1NaK in the cells was not significantly altered. On the other hand, the expres...Continue Reading
References
Aug 24, 1970·Biochemical and Biophysical Research Communications·A Yoda, L E Hokin
Mar 23, 1994·Biochimica Et Biophysica Acta·K BambergH F Helander
May 23, 1997·The Journal of Biological Chemistry·P E BickelH F Lodish
Jun 5, 1997·Nature·K Simons, E Ikonen
Apr 23, 1999·The Journal of Biological Chemistry·D VolonteM P Lisanti
May 29, 1999·The Journal of Biological Chemistry·D B MountG Gamba
Sep 10, 1999·The Journal of Biological Chemistry·M FlagellaG E Shull
Dec 22, 1999·The American Journal of Physiology·J E RaceE J Holtzman
Mar 14, 2001·Neuroscience·M M PearsonE Delpire
Aug 3, 2001·The Journal of Physiology·H RossmannU Seidler
Sep 21, 2001·The Journal of Cell Biology·M CarmosinoG Valenti
Dec 4, 2001·The Journal of Physiology·X LiM Donowitz
Mar 23, 2002·The Journal of Biological Chemistry·Tohru KimuraShinji Asano
Jul 26, 2002·The Journal of Biological Chemistry·Satomi TeramachiKazuya Taniguchi
Jun 20, 2003·The Journal of Physiology·Hideki SakaiNoriaki Takeguchi
Feb 14, 2004·The Journal of Biological Chemistry·Haojie WangZijian Xie
Mar 30, 2004·Nature Cell Biology·Roman PolishchukJennifer Lippincott-Schwartz
May 5, 2004·The Journal of Biological Chemistry·Lara R GawenisGary E Shull
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz
Nov 27, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Keiko IkedaKiyoshi Kawakami
Dec 17, 2004·Molecular Pharmacology·Peter KometianiAmir Askari
Feb 16, 2005·The Journal of Membrane Biology·N C AdragnaP K Lauf
Mar 15, 2005·The Journal of Biological Chemistry·Toshiaki ImagawaKazuya Taniguchi
Mar 25, 2005·Physiological Reviews·Gerardo Gamba
Aug 13, 2005·The Journal of Biological Chemistry·Daniel LingwoodJames S Ballantyne
Aug 19, 2005·American Journal of Physiology. Cell Physiology·Corina BalutDanny Jans
Nov 4, 2005·Molecular Biology of the Cell·Jiang TianZi-Jian Xie
May 2, 2006·The Journal of Biological Chemistry·Rakhilya MurtazinaXuhang Li
Feb 14, 2007·The Journal of Biological Chemistry·Man LiangZi-Jian Xie
Feb 17, 2007·European Journal of Pharmacology·Takuto FujiiHideki Sakai
Citations
Sep 21, 2011·The Journal of Physiological Sciences : JPS·Takuto FujiiHideki Sakai
Aug 6, 2009·Current Opinion in Nephrology and Hypertension·Xiaoyan WangPedro A Jose
Aug 5, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Peter M PiermariniKlaus W Beyenbach
Apr 15, 2011·Journal of Lipids·Katie D Hickey, Mary M Buhr
Dec 14, 2011·BMC Research Notes·Anna-Maria Hartmann, Hans Gerd Nothwang
Jun 2, 2011·Biological & Pharmaceutical Bulletin·Takuto FujiiHideki Sakai
Aug 11, 2011·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Takuto FujiiHideki Sakai
Sep 11, 2013·PloS One·André DagenaisYves Berthiaume
Nov 6, 2013·FEBS Letters·Takuto FujiiHideki Sakai
Jun 27, 2012·Biochemical and Biophysical Research Communications·Kyosuke FujitaHideki Sakai
Aug 10, 2010·Biochemical and Biophysical Research Communications·Takuto FujiiHideki Sakai
May 8, 2010·European Journal of Pharmacology·Kazuto ShibuyaKazuhiro Tsukada
Aug 21, 2015·Chemistry and Physics of Lipids·Elisabeth A CalhoonJoseph B Williams
Aug 19, 2009·Journal of Neurochemistry·Anna-Maria HartmannHans Gerd Nothwang
Nov 17, 2009·Biology of the Cell·Monica CarmosinoMaria Svelto
Mar 1, 2016·Biochimica Et Biophysica Acta·Takuto FujiiHideki Sakai
Aug 19, 2015·European Journal of Pharmacology·Takuto FujiiHideki Sakai
Jul 27, 2012·Epilepsy & Behavior : E&B·Antônio-Carlos G De AlmeidaFulvio A Scorza
Oct 3, 2015·Cerebral Cortex·Goran SedmakMiloš Judaš
Jun 26, 2017·Neurobiology of Disease·Melissa BowermanFrédérique Scamps
Jan 20, 2019·The Journal of Physiology·A P GarneauP Isenring
Oct 4, 2013·American Journal of Physiology. Renal Physiology·Zesergio MeloGerardo Gamba
Oct 16, 2014·American Journal of Physiology. Cell Physiology·Peter K LaufNorma C Adragna
Nov 6, 2008·The Journal of Biological Chemistry·Takuto FujiiHideki Sakai
Aug 18, 2017·American Journal of Physiology. Cell Physiology·A P GarneauP Isenring
Oct 19, 2020·Cellular and Molecular Gastroenterology and Hepatology·Tyler J CaronSusan J Hagen
Dec 15, 2020·Science Advances·Yuan XieJiangtao Guo