PMID: 11913461Mar 27, 2002Paper

K-Cl co-transport: immunocytochemical and functional evidence for more than one KCC isoform in high K and low K sheep erythrocytes

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
P K LaufNorma C Adragna

Abstract

K-Cl co-transport (COT) is significantly higher in low K (LK), L-antigen (L) positive, than in high K (HK), M-antigen (M) positive, sheep red blood cells (SRBCs) and is inhibited by sheep allo-anti-L1 antibody. To answer the question of whether this difference in K-Cl co-transport activity resides at the level of the transporter or its regulation, a combined immunocytochemical and functional approach was taken. At least four isoforms of K-Cl COT encoded by different KCC genes are known, with 12 transmembrane domains and cytoplasmic C- and N-terminal domains (Ctd and Ntd, respectively). Polyclonal anti-rat (rt)KCC1 antibodies against a fusion peptide with 77 amino acids from the Ctd of rtKCC1 and anti-human (h)KCC3 against an 18-aa peptide from the Ntd of hKCC3, were prepared in rabbits (rb). Two distinctly separate protein bands of 180 and 145 kDa molecular mass were detected in hemoglobin-free ghosts from RBCs of two LK (one homozygous LL and one heterozygous LM) and one HK (homozygous MM) sheep by Western blots with rb anti-rtKCC1 and rb anti-hKCC3. Confocal microscopy showed specific immunostaining of KCC1 with rb anti-rtKCC1, and of KCC3 with rb anti-hKCC3, in white ghosts from both LK and HK SRBCs. To test the functional h...Continue Reading

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Citations

Feb 8, 2006·The Journal of Membrane Biology·J E Capó-AponteP S Reinach
May 25, 2005·Experimental Hematology·Scott C CrableKathleen P Anderson
Jul 8, 2011·The Journal of Biological Chemistry·Dao PanClinton H Joiner
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Dec 14, 2011·BMC Research Notes·Anna-Maria Hartmann, Hans Gerd Nothwang
Oct 24, 2007·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Norma C Adragna, Peter K Lauf
Jul 6, 2015·Trends in Molecular Medicine·Kristopher T KahleEric Delpire
Jul 29, 2004·The Journal of Biological Chemistry·Charles F SimardPaul Isenring
Feb 16, 2005·The Journal of Membrane Biology·N C AdragnaP K Lauf
Feb 12, 2009·Journal of Cellular Physiology·Marc J BergeronPaul Isenring
Jul 29, 2005·American Journal of Physiology. Renal Physiology·Adriana MercadoDavid B Mount
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jin J ZhangPeter K Lauf
Mar 2, 2006·Experimental Animals·Hideharu OchiaiHiroshi Fujise
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P K LaufG A Rouleau
May 10, 2003·Pflügers Archiv : European journal of physiology·P MercirisF Giraud
Aug 18, 2017·American Journal of Physiology. Cell Physiology·A P GarneauP Isenring
Nov 18, 2005·The Journal of Biological Chemistry·Adriana MercadoDavid B Mount

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