PMID: 11607404Jun 1, 1993Paper

K+-conducting ion channel of the chloroplast inner envelope: functional reconstitution into liposomes

Proceedings of the National Academy of Sciences of the United States of America
X WangJ S Peters

Abstract

Potassium flux between the chloroplast stroma and cytoplasm is known to be indirectly linked to H+ countertransport and, hence, stromal pH and photosynthetic capacity. The specific molecular mechanism that facilitates K+ flux across the chloroplast envelope is not known and has been a source of controversy for well over a decade. The objective of this study was to elucidate the nature of this envelope protein. To this end, solubilized protein in detergent extracts of purified chloroplast inner envelope vesicles was reconstituted into artificial liposomes, and cation fluxes into these proteoliposomes were measured. Results of inhibitor studies and counterflux experiments indicated that a K+-conducting ion channel was solubilized and functionally reconstituted into the proteoliposomes. This transport protein may be a nonspecific monovalent cation channel. This report represents a direct demonstration of ion channel activity associated with the limiting (inner) membrane of the chloroplast envelope.

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Citations

Oct 26, 2005·The Journal of Membrane Biology·I I PottosinS Shabala
Aug 1, 1996·Current Opinion in Cell Biology·S M Assmann, L L Haubrick
May 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hans-Henning KunzJulian I Schroeder
Mar 15, 2016·Frontiers in Plant Science·Ricarda HöhnerKees Venema
Jan 13, 2006·Current Biology : CB·Elizabeth S Haswell, Elliot M Meyerowitz
May 4, 2012·Biochimica Et Biophysica Acta·María Nieves Aranda-SiciliaKees Venema
Apr 28, 1995·The Journal of Biological Chemistry·B Fuks, F Homblé
Oct 29, 2020·The New Phytologist·María Nieves Aranda SiciliaKees Venema

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