PMID: 2493742Mar 1, 1989Paper

K-independent Na-Cl cotransport in bovine tracheal epithelial cells

The American Journal of Physiology
M W Musch, M Field

Abstract

Uptakes of 22Na, 36Cl, and 86Rb into isolated bovine tracheal epithelial cells were measured in the presence and absence of 10 microM bumetanide. Preincubation with ouabain (0.5 mM) did not alter initial rates of Na and Cl uptakes but prolonged from 0.5 or less to 2 min the period in which Na uptake is linear with time. Initial rates of bumetanide-inhibitable Na and Cl uptakes (influxes), measured for 2 min in identical solutions, were similar in magnitude (bumetanide-sensitive Cl influx/bumetanide-sensitive Na influx = 1.2). Omission of K did not affect bumetanide-sensitive Na or Cl influx. Cl influx was not affected by 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid. Amiloride (0.1 mM) partially inhibited the bumetanide-insensitive Na influx but had no effect on the bumetanide-sensitive Na influx. Rb influx was not affected by bumetanide but was markedly reduced by ouabain and slightly reduced by Ba, the combination being additive. Half-maximally inhibitory concentration values for inhibition of Cl influx by 4 sulfamoylbenzoic acid derivatives were as follows (in mumol/l): 0.125 benzmetanide; 0.64 bumetanide; 16 piretanide; and 26 furosemide. Affinities of Na and Cl for the bumetanide-inhibitable cotransport process ...Continue Reading

References

Aug 1, 1979·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·F J Al-Bazzaz, E Cheng
Mar 1, 1986·The Journal of Physiology·J DurandF Schoenenweid
Jan 1, 1985·The Journal of Membrane Biology·A C Hall, J C Ellory
Jan 1, 1981·Annals of the New York Academy of Sciences·R A FrizzellP L Smith

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Citations

Apr 1, 1991·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·C Lehmann, J Durand
Jan 5, 2000·Physiological Reviews·J M Russell
Oct 1, 1994·The American Journal of Physiology·M Haas
Aug 1, 1995·Current Opinion in Cell Biology·J A Payne, B Forbush
Jul 21, 1991·Journal of Theoretical Biology·M Duszyk, A S French

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