K-ras mutation analysis of residual liquid-based cytology specimens from endoscopic ultrasound-guided fine needle aspiration improves cell block diagnosis of pancreatic ductal adenocarcinoma

PloS One
Yoko Sekita-HatakeyamaChiho Ohbayashi

Abstract

Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) technology is widely used for the diagnosis of pancreatic masses. However, in some cases, inadequate tissue volume or difficulty of morphological diagnosis are constraining factors for adequate cytopathological evaluation. K-ras mutation is the most frequently acquired genetic abnormality, occurring in approximately 90% of all patients with pancreatic ductal adenocarcinoma (PDAC). In the present study, the clinical utility of residual liquid-based cytology (LBC) specimens obtained using EUS-FNA for K-ras mutation analysis was evaluated. In this study, 81 patients with pancreatic lesions were examined. The cell block (CB) specimens separated from EUS-FNA samples were morphologically evaluated by hematoxylin-eosin (HE) staining. Final diagnoses were confirmed by CB specimens, surgical resection specimens, diagnostic imaging, and clinical follow-up. Genomic DNA of residual LBC specimens stored at 4°C for several months were extracted and assessed for K-ras mutations using a fluorescence resonance energy transfer-based preferential homoduplex formation assay. K-ras mutation analysis using residual LBC samples was successful in all cases. The sensitivity, specificity, and...Continue Reading

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Citations

Nov 14, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·Yoko MatsudaTomio Arai
Feb 2, 2020·Nature Reviews. Gastroenterology & Hepatology·Louis BuscailPierre Cordelier
Jun 30, 2019·Cancers·Etienne BuscailLouis Buscail

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Methods Mentioned

BETA
fluorescence
PCR
electrophoresis

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