PMID: 9428484Jan 15, 1998Paper

K-ras mutations in the duodenal fluid of patients with pancreatic carcinoma

Cancer
R E WilentzR J Slebos

Abstract

Many patients with carcinoma of the pancreas die because their disease is not detected until late in its course. Methods that detect these cancers earlier will improve patient outcome. Over 80% of pancreatic carcinomas contain mutations in codon 12 of the K-ras gene. Screening duodenal fluid for these mutations may lead to early detection of these cancers and assist in establishing a diagnosis of pancreatic carcinoma. Polymerase chain reaction (PCR), with and without restriction enzyme-mediated mutant enrichment, was performed on DNA isolated from duodenal fluid specimens from 61 patients who underwent pancreaticoduodenectomy (Whipple's operation) for either periampullary cancer or a benign condition of the pancreas. Representative sections of pancreas pathology (primary carcinoma, benign tumor, or chronic pancreatitis) from the patients with duodenal fluid specimens containing amplifiable DNA were also analyzed for K-ras mutations. Wild-type and mutant K-ras were detected by hybridization of the PCR products with K-ras codon 12 mutant and wild-type specific probes. Seven of the 61 duodenal fluid specimens contained DNA that did not amplify. Thirteen (24% of the 54 duodenal fluid specimens with amplifiable DNA and 21% of the to...Continue Reading

References

Apr 1, 1979·Cancer·S KozukaM Takeuchi
Jan 1, 1992·Gastroenterology·N R LemoineG Klöppel
Feb 13, 1992·The New England Journal of Medicine·A L Warshaw, C Fernández-del Castillo
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Jun 15, 1989·International Journal of Cancer. Journal International Du Cancer·K GrünewaldC R Bartram
Aug 1, 1989·Human Pathology·M ShimizuY Saitoh
Jul 16, 1985·Biochemical and Biophysical Research Communications·S E GoelzB Vogelstein
Aug 25, 1988·Nucleic Acids Research·V T SmitJ L Bos
Jan 1, 1987·Annual Review of Biochemistry·M Barbacid
Aug 1, 1995·Annals of Internal Medicine·P BerthélemyL Pradayrol
Mar 1, 1994·Pancreas·B K SchaefferD S Longnecker
Sep 1, 1993·Japanese Journal of Cancer Research : Gann·H WatanabeT Wakabayashi
Jan 1, 1996·CA: a Cancer Journal for Clinicians·S L ParkerP A Wingo
Jun 1, 1996·Journal of Clinical Pathology·C H ChungR J Slebos

❮ Previous
Next ❯

Citations

Apr 17, 2012·Asian Journal of Andrology·Kathryn M WilsonLorelei A Mucci
Jul 23, 1999·Annals of the New York Academy of Sciences·D S Longnecker
Mar 23, 2005·Nature Methods·Chanjuan ShiJames R Eshleman
Oct 7, 2003·Annual Review of Genomics and Human Genetics·Donna E HanselRalph H Hruban
Feb 10, 2016·Molecular Oncology·Francesca RivaFrancois-Clement Bidard
Oct 10, 2013·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Shang-Long LiuTai-Ping Zhang
Jan 31, 2003·Pathology·Pauline de la M HallPhilippus P C Bornman
Jul 13, 2013·Chembiochem : a European Journal of Chemical Biology·Kiyohiko KawaiAtsushi Maruyama
Jun 19, 2016·Bioelectrochemistry·Tania García-MendiolaEncarnación Lorenzo
Jul 11, 2012·Journal of the American Chemical Society·Hsin-Chih YehJames H Werner
Oct 1, 2016·European Journal of Medicinal Chemistry·Vivek AsatiSanjay Kumar Bharti
Aug 30, 2000·Journal of Surgical Oncology·M GogginsR Hruban
Jul 9, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Michael Goggins
Mar 8, 2005·Clinics in Laboratory Medicine·Asif KhalidKevin McGrath
Mar 29, 2001·Current Gastroenterology Reports·H T LynchR M Fusaro
Jun 14, 2000·Breast Cancer Research and Treatment·M MiyazakiM Monden
Nov 11, 1999·The American Journal of Surgical Pathology·R E WilentzR H Hruban
Oct 7, 2006·Current Opinion in Gastroenterology·R H HrubanS E Kern
Oct 6, 2016·Oncotarget·Florence Le Calvez-KelmGhislaine Scelo
Feb 3, 2000·Journal of Epidemiology and Community Health·M PortaF X Real
Aug 15, 2009·International Journal of Cancer. Journal International Du Cancer·Antoine ItalianoFlorence Pedeutour
Oct 14, 2010·Cell Biochemistry and Function·Peter B Gahan, Maurice Stroun
Feb 28, 2017·International Journal of Cancer. Journal International Du Cancer·Bin ZhouHan-Xiang Zhan
Sep 1, 2016·Chemical Science·Tania García-MendiolaEncarnación Lorenzo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.