Abstract
Pancreaticobiliary maljunction (PBM), an anomalous union of the pancreatic duct with the common bile duct, has frequently been shown to be associated with biliary carcinoma. However, the mechanism of carcinogenesis is unknown. Mutations of the K-ras oncogene were examined in cancerous and noncancerous biliary tract epithelium of 20 patients with PBM by an extraction of DNA from surgically resected histologic specimens. DNA was analyzed by a polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) method and direct sequencing. An abnormally mobilized DNA band was detected not only in cancerous epithelium but also in hyperplastic, metaplastic, and inflammatory epithelium of the gallbladder and/or common bile duct in patients with PBM. Among the biliary epithelium of patients with PBM, point mutation of K-ras oncogenes were detected in 4 of 5 (80%) cancerous epithelium, 7 of 12 (58%) hyperplastic and metaplastic epithelium, and 8 of 18 (44%) inflammatory epithelium, whereas no point mutation of the K-ras oncogene was detected in the gallbladder epithelium in 3 control patients without PBM. Direct sequence analysis of the K-ras oncogene revealed the mutation at codon 12 substituting the wild-type glycine (GGT) f...Continue Reading
References
Apr 1, 1977·AJR. American Journal of Roentgenology·K KimuraK Okuda
Jan 1, 1992·Molecular Carcinogenesis·H TsudaS Hirohashi
Mar 1, 1992·Cancer·M TadaM Ohto
Dec 1, 1991·Annals of Surgery·K MotojimaH Shiku
Sep 1, 1991·The Keio Journal of Medicine·T FunabikiM Ochiai
Feb 1, 1991·Cancer·M TadaM Ohto
Jan 1, 1991·Analytical Biochemistry·Y SuzukiK Hayashi
Feb 1, 1990·Japanese Journal of Cancer Research : Gann·Y NagataH Shiku
Sep 1, 1990·Cancer·M TadaK Isono
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
May 3, 1987·Nature·K ForresterM Perucho
May 20, 1988·Cell·C AlmogueraM Perucho
Jul 1, 1989·Japanese Journal of Cancer Research : Gann·M MariyamaS Nishimura
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Jun 15, 1989·International Journal of Cancer. Journal International Du Cancer·K GrünewaldC R Bartram
Nov 1, 1989·Genomics·M OritaK Hayashi
Sep 1, 1988·The New England Journal of Medicine·B VogelsteinJ L Bos
Aug 25, 1988·Nucleic Acids Research·V T SmitJ L Bos
Aug 1, 1983·Archives of Surgery·C R VoylesL H Blumgart
Oct 1, 1994·Gastroenterology·M WatanabeT Miyazaki
Jun 1, 1994·Cancer·M ImaiK Ogawa
Citations
Nov 27, 2008·World Journal of Gastroenterology : WJG·Terumi KamisawaKouji Tsuruta
Jun 19, 2008·Journal of Pediatric Surgery·Shigeru OnoNaomi Iwai
Apr 13, 2013·Journal of Hepato-biliary-pancreatic Sciences·Yuji MorineHiroshi Shimada
Mar 17, 2016·Biochimica Et Biophysica Acta·Jaime A EspinozaJuan C Roa
Apr 27, 2016·Journal of Pediatric Surgery·Enrico La PergolaMark Davenport
Mar 6, 1999·Journal of Hepatology·M SackmannT Helmberger
Oct 7, 2003·Annals of Surgical Oncology·Michael G HouseAnirban Maitra
Mar 16, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Takahiro InoueMasazumi Tsuneyoshi
Jan 1, 2013·ISRN Radiology·Abhishek VijayakumarB S Shivaswamy
Sep 3, 2004·Nature Reviews. Cancer·Ignacio I Wistuba, Adi F Gazdar
Jun 11, 1999·The American Journal of Gastroenterology·K HanadaG Kajiyama
Feb 13, 2019·Carcinogenesis·Masako OchiaiYoshitaka Hippo
Nov 3, 2001·European Journal of Gastroenterology & Hepatology·K OtaniM Tanaka
Jun 10, 2005·Carcinogenesis·Akihiko TsuchidaTatsuya Aoki
Apr 20, 2017·Oncotarget·Mengdan LiPeng Hou
Apr 10, 2003·Langenbeck's Archives of Surgery·Yoshiro MatsumotoKohichi Suda
Oct 24, 2014·World Journal of Gastroenterology : WJG·Narongsak Rungsakulkij, Paisarn Boonsakan
Oct 31, 2006·World Journal of Gastroenterology : WJG·Jin Kan SaiBunsei Nobukawa
Nov 4, 2017·Surgical Case Reports·Haruki MoriMasaji Tani
Jan 9, 2013·International Surgery·Kazuhiro TakahashiNobuhiro Ohkohchi
Mar 27, 2001·European Journal of Clinical Investigation·P MüllerJ M Löhr
Feb 16, 2010·The British Journal of Surgery·T KosakaT Kanematsu
Apr 8, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S G BarretoA Chaudhary