K-ras point mutations in cancerous and noncancerous biliary epithelium in patients with pancreaticobiliary maljunction

Cancer
T MatsubaraI Hirono

Abstract

Pancreaticobiliary maljunction (PBM), an anomalous union of the pancreatic duct with the common bile duct, has frequently been shown to be associated with biliary carcinoma. However, the mechanism of carcinogenesis is unknown. Mutations of the K-ras oncogene were examined in cancerous and noncancerous biliary tract epithelium of 20 patients with PBM by an extraction of DNA from surgically resected histologic specimens. DNA was analyzed by a polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) method and direct sequencing. An abnormally mobilized DNA band was detected not only in cancerous epithelium but also in hyperplastic, metaplastic, and inflammatory epithelium of the gallbladder and/or common bile duct in patients with PBM. Among the biliary epithelium of patients with PBM, point mutation of K-ras oncogenes were detected in 4 of 5 (80%) cancerous epithelium, 7 of 12 (58%) hyperplastic and metaplastic epithelium, and 8 of 18 (44%) inflammatory epithelium, whereas no point mutation of the K-ras oncogene was detected in the gallbladder epithelium in 3 control patients without PBM. Direct sequence analysis of the K-ras oncogene revealed the mutation at codon 12 substituting the wild-type glycine (GGT) f...Continue Reading

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Citations

Nov 27, 2008·World Journal of Gastroenterology : WJG·Terumi KamisawaKouji Tsuruta
Apr 13, 2013·Journal of Hepato-biliary-pancreatic Sciences·Yuji MorineHiroshi Shimada
Mar 17, 2016·Biochimica Et Biophysica Acta·Jaime A EspinozaJuan C Roa
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Apr 10, 2003·Langenbeck's Archives of Surgery·Yoshiro MatsumotoKohichi Suda
Oct 24, 2014·World Journal of Gastroenterology : WJG·Narongsak Rungsakulkij, Paisarn Boonsakan
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Apr 8, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S G BarretoA Chaudhary

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