K+ transport and capacitance of the basolateral membrane of the larval frog skin

The American Journal of Physiology
S D HillyardW Van Driessche

Abstract

Skin from larval bullfrogs was mounted in an Ussing-type chamber in which the apical surface was bathed with a Ringer solution containing 115 mM K+ and the basolateral surface was bathed with a Ringer solution containing 115 mM Na+. Ion transport was measured as the short-circuit current (Isc) with a low-noise voltage clamp, and skin resistance (Rm) was measured by applying a direct current voltage pulse. Membrane impedance was calculated by applying a voltage signal consisting of 53 sine waves to the command stage of the voltage clamp. From the ratio of the Fourier-transformed voltage and current signals, it was possible to calculate the resistance and capacitance of the apical and basolateral membranes of the epithelium (Ra and Rb, Ca and Cb, respectively). With SO4(2-) as the anion, Rm decreased rapidly within 5 min following the addition of 150 U/ml nystatin to the apical solution, whereas Isc increased from 0.66 to 52.03 microA/cm2 over a 60-min period. These results indicate that nystatin becomes rapidly incorporated into the apical membrane and that the increase in basolateral K+ permeability requires a more prolonged time course. Intermediate levels of Isc were obtained by adding 50, 100, and 150 U/ml nystatin to the ap...Continue Reading

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Citations

Jul 3, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Willy Van DriesscheKwang-Jin Kim
Dec 5, 2013·Biological Reviews of the Cambridge Philosophical Society·Iain S HaslamRalf Paus
Oct 4, 2011·General and Comparative Endocrinology·Edison S M CarvalhoDeborah M Power

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