Abstract
Kaempferol, a natural flavonoid, has the beneficial effects of preserving pancreatic β-cell mass and function, but its action on β-cell lipid metabolism still remains elusive. Recently, autophagy has been reported to play a major role in lipid metabolism in various cell types, but its role in pancreatic β-cell's lipid metabolism is rarely reported. Here, we investigated the role of kaempferol-induced autophagy in inhibition of lipid stores, ER stress and β-cell dysfunction in palmitic acid-challenged RIN-5F cells and isolated pancreatic islets. The lipid-lowering effect of kaempferol was determined by Oil Red O staining, triglyceride assay, BODIPY labeling, RT-PCR and immunoblot analysis of PLIN2 (the lipid droplet coat protein) expression. Further, the involvement of AMPK/mTOR-mediated lipophagy was established by pharmacological and genetic inhibitors of autophagy and AMPK. The co-localization studies of lipid droplets with autophagosomes/lysosomes by BODIPY-MDC-LysoTracker co-staining, LC3/BODIPY labeling and LC3/PLIN2 double immunolabeling further strengthened the findings. Kaempferol treatment exhibited decreased lipid stores and increased co-localization of lipid droplets with autophagosomes and lysosomes in palmitic acid...Continue Reading
Citations
Jul 27, 2018·Current Drug Targets·Linghuan LiHanbing Li
Dec 13, 2019·Phytotherapy Research : PTR·Milad AshrafizadehSaeed Samarghandian
Jun 22, 2019·Current Vascular Pharmacology·Xiaoxue LiHui Huang
Sep 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sonia Marlen Escandón-RiveraAdolfo Andrade-Cetto
May 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peipei YinYujun Liu
Jul 18, 2018·International Journal of Molecular Sciences·Zhuo Mao, Weizhen Zhang
May 21, 2019·Frontiers in Physiology·Yan WangPing Li
Oct 22, 2019·Frontiers in Physiology·Xiangming WangWei Jing Liu
Apr 15, 2020·Journal of Cardiovascular Translational Research·Lei QiaoYun Zhang
Aug 2, 2020·Acta Pharmacologica Sinica·Jun-Shang HuangHe-Yao Wang
Oct 9, 2020·Food & Function·Jing LuShuang Guan
Oct 20, 2020·Molecular Biology Reports·Rutusmita MishraPartha Roy
Dec 16, 2020·Critical Reviews in Food Science and Nutrition·Han-Ning WangMin Du
Dec 5, 2020·Journal of Diabetes Research·Yuyang WangYongli Zhan
Mar 6, 2021·Life Sciences·Hui LiSuixin Liu
Mar 28, 2021·Free Radical Biology & Medicine·Somesh BanerjeePartha Roy
May 28, 2021·Experimental Eye Research·Huiwen TianJing Xu
Jun 10, 2021·Food & Function·Rutusmita MishraPartha Roy
Jul 9, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Mingyue HuangDan Yan
Jul 3, 2021·Antioxidants·Jen-Ying HsuJing-Hsien Chen
Aug 3, 2021·Frontiers in Endocrinology·Kexin WangYu Sun
Aug 28, 2021·International Journal of Molecular Sciences·Fangfang TieHonglun Wang
Nov 14, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Debasish MohapatraShantibhusan Senapati