Abstract
Gastric cancer remains a serious threat to human health worldwide. Kaempferol is a plant-derived flavonoid compound with a wide range of pharmacological activities. This study aimed to investigate the effects of kaempferol on gastric cancer SNU-216 cell proliferation, apoptosis, and autophagy, as well as underlying potential mechanisms. Viability, proliferation, and apoptosis of SNU-216 cells after kaempferol treatment were evaluated using cell counting kit-8 assay, 5-btomo-2'-deoxyuridine incorporation assay, and annexin V-FITC/PI staining, respectively. Quantitative reverse transcription PCR was performed to measure the mRNA expressions of cyclin D1 and microRNA-181a (miR-181a) in SNU-216 cells. Cell transfection was used to down-regulate the expression of miR-181a. The protein expression levels of cyclin D1, bcl-2, bax, caspase 3, caspase 9, autophagy-related gene 7, microtubule-associated protein 1 light chain 3-I (LC3-I), LC3-II, Beclin 1, p62, mitogen-activated protein kinase (MAPK), extracellular regulated protein kinases (ERK), and phosphatidylinositol 3 kinase (PI3K) in SNU-216 cells were detected using western blotting. Results showed that kaempferol significantly suppressed SNU-216 cell viability and proliferation bu...Continue Reading
References
Aug 23, 2005·Gene·Fabrício F Costa
Oct 4, 2005·The Journal of Clinical Investigation·Beth Levine, Junying Yuan
Dec 7, 2007·Genes & Development·Gábor JuhászThomas P Neufeld
Feb 16, 2008·Drug Discovery Today·Arvind Saklani, Samuel K Kutty
Mar 20, 2010·Methods in Molecular Biology·Shahar Ish-Shalom, Amnon Lichter
Feb 18, 2012·Nano Letters·Francesco AietaFederico Capasso
Apr 18, 2013·International Journal of Oncology·Wen-Wen HuangJai-Sing Yang
May 4, 2013·Gastroenterology Clinics of North America·Roopma WadhwaJaffer A Ajani
Aug 1, 2013·Human & Experimental Toxicology·H YanY Xiao
Aug 7, 2013·International Journal of Pharmaceutics·Eduardo AnsorenaAnne des Rieux
Mar 4, 2014·World Journal of Gastroenterology : WJG·Michele OrdituraFerdinando De Vita
Apr 5, 2014·Molecular Carcinogenesis·Qiang DangLei Li
Jul 19, 2014·Anti-cancer Drugs·Xiaofang XingJiafu Ji
Jan 1, 2012·MicroRNA·Jyoti DuggalXuening Wang
Sep 6, 2014·Current Pharmaceutical Biotechnology·Yusuf Tutar
Sep 14, 2014·Journal of Cancer Research and Clinical Oncology·Rong-Kun LiZhi-Gang Zhang
Oct 14, 2014·International Journal of Oncology·Xiyan XiaoYanping Zhang
Oct 25, 2014·Oncology Research and Treatment·Xiaofang GengCunshuan Xu
Dec 3, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Francesca PistollatoMaurizio Battino
Dec 9, 2014·Methods : a Companion to Methods in Enzymology·Peidu Jiang, Noboru Mizushima
Dec 17, 2014·Oncology Reports·Haibin SongYingwei Xue
Feb 24, 2015·Oncology Research·Feng LinWeidong Mao
Apr 15, 2015·International Immunopharmacology·Fang LinBin Li
Apr 29, 2015·Laboratory Medicine·Hui Wang, Chang-Ping Men
May 23, 2015·Molecular Medicine Reports·Du-Guan Fu
Jun 7, 2015·Molecular and Cellular Biochemistry·Kwangho KimHara Kang
Aug 25, 2015·Neoplasia : an International Journal for Oncology Research·Eunji JoByung-Chul Kim
Feb 18, 2016·The Journal of Nutritional Biochemistry·Seung-Hee KimKyung-Chul Choi
Feb 26, 2016·World Journal of Gastrointestinal Oncology·Jahanarah KhatoonKashi Nath Prasad
Mar 5, 2016·Animal : an International Journal of Animal Bioscience·I M AparicioJ A Tapia
May 14, 2016·PloS One·Jungwhoi Lee, Jae Hoon Kim
Oct 4, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Shuo Qie, J Alan Diehl
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Nov 16, 2017·Oncotarget·Haiqing GuoFeng Ren
Citations
Nov 14, 2019·Current Drug Delivery·Milad AshrafizadehSaeed Samarghandian
Dec 13, 2019·Phytotherapy Research : PTR·Milad AshrafizadehSaeed Samarghandian
Sep 16, 2020·International Journal of Molecular Sciences·Monica BenvenutoRoberto Bei
Mar 10, 2020·BioMed Research International·Li-Qun RenYang Zhang
Mar 20, 2020·Toxicology Research·Magdalena KluskaKatarzyna Woźniak
May 12, 2020·Frontiers in Pharmacology·Sisi CaoHongwei Guo
Jul 28, 2020·Cancers·Andrea M TomkoDenis J Dupré
Dec 5, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yi-Ding YuYi-Tao Xue
May 13, 2021·International Journal of General Medicine·Ting XiuFan-Bo Jing
Jun 23, 2021·Environmental Toxicology·Po-Chung JuChao-Bin Yeh
Aug 22, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mohammad Hossein PourhanifehHamed Mirzaei