Kaempferol suppresses human gastric cancer SNU-216 cell proliferation, promotes cell autophagy, but has no influence on cell apoptosis

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Fan Zhang, Cuimei Ma

Abstract

Gastric cancer remains a serious threat to human health worldwide. Kaempferol is a plant-derived flavonoid compound with a wide range of pharmacological activities. This study aimed to investigate the effects of kaempferol on gastric cancer SNU-216 cell proliferation, apoptosis, and autophagy, as well as underlying potential mechanisms. Viability, proliferation, and apoptosis of SNU-216 cells after kaempferol treatment were evaluated using cell counting kit-8 assay, 5-btomo-2'-deoxyuridine incorporation assay, and annexin V-FITC/PI staining, respectively. Quantitative reverse transcription PCR was performed to measure the mRNA expressions of cyclin D1 and microRNA-181a (miR-181a) in SNU-216 cells. Cell transfection was used to down-regulate the expression of miR-181a. The protein expression levels of cyclin D1, bcl-2, bax, caspase 3, caspase 9, autophagy-related gene 7, microtubule-associated protein 1 light chain 3-I (LC3-I), LC3-II, Beclin 1, p62, mitogen-activated protein kinase (MAPK), extracellular regulated protein kinases (ERK), and phosphatidylinositol 3 kinase (PI3K) in SNU-216 cells were detected using western blotting. Results showed that kaempferol significantly suppressed SNU-216 cell viability and proliferation bu...Continue Reading

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Citations

Nov 14, 2019·Current Drug Delivery·Milad AshrafizadehSaeed Samarghandian
Dec 13, 2019·Phytotherapy Research : PTR·Milad AshrafizadehSaeed Samarghandian
Sep 16, 2020·International Journal of Molecular Sciences·Monica BenvenutoRoberto Bei
Dec 5, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yi-Ding YuYi-Tao Xue
May 13, 2021·International Journal of General Medicine·Ting XiuFan-Bo Jing
Aug 22, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mohammad Hossein PourhanifehHamed Mirzaei

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Methods Mentioned

BETA
reverse
transfection
flow cytometry
protein assay
reverse transcription PCR

Software Mentioned

FlowJo
Quantity One
GraphPad

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