PMID: 9556030Apr 29, 1998Paper

Kainate-induced apoptosis correlates with c-Jun activation in cultured cerebellar granule cells

Journal of Neuroscience Research
N S CheungS F Giardina

Abstract

We have investigated the involvement of c-Jun in cell death induced by exposure of primary cultures of murine cerebellar granule cells to the glutamate receptor agonist kainate (KA) and evaluated its possible use as a marker for apoptosis. Using cerebellar granule cell neurones from postnatal day 7 mice, we found that 1 hr exposure to KA (1-1000 microM) induced a concentration-dependent neuronal cell death with characteristic apoptotic morphology, including cell shrinkage, neurite blebbing and DNA fragmentation. In addition KA-induced a concentration-dependent expression of c-Jun mRNA and protein as determined by in situ hybridization and immunocytochemistry respectively. DNA fragmentation was detected using terminal transferase-mediated nick-end (TUNEL) labelling and agarose gel electrophoresis. KA-induced cell death was significantly attenuated by the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 50 microM), which shifted the concentration-response curve significantly rightward. The number of apoptotic cell bodies, determined by TUNEL, was also reduced by CNQX (50 microM), with only 15-20% of neurones staining positive after exposure to 1mM KA. In addition, the number of positively stained cells for...Continue Reading

Citations

Nov 9, 2010·Biochimica Et Biophysica Acta·Salvatore IncontroMagdalena Torres
Mar 6, 2002·British Journal of Pharmacology·Ester VerdaguerAntoni Camins
Mar 20, 2009·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak, William L Lanier
Jun 19, 2010·Journal of Neurochemistry·Samantha P FernandesStephen R Robinson
Mar 6, 2009·Journal of Neural Transmission·Konstantinos SpiliopoulosAda Mitsacos
May 1, 2010·Neurotoxicity Research·Glenda M BishopStephen R Robinson
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Jun 24, 2004·Journal of Neuroscience Research·Steven A SmithSarah J Roberts-Thomson
Sep 7, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gennaro GiordanoLucio G Costa
Mar 26, 2008·Journal of Neurosurgical Anesthesiology·Li WangYan Nan Hang
Feb 16, 2006·Journal of Neuroscience Research·Yasunari MunemasaSatoki Ueno
Jun 17, 2000·Progress in Neurobiology·K J BanasiakG G Haddad
Jan 31, 2008·Journal of Neuroinflammation·Piali MukherjeeGiulio Maria Pasinetti
Mar 15, 2011·Neurochemistry International·Theresa N DangGlenda M Bishop

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis