Kainate seizures increase nociceptin/orphanin FQ release in the rat hippocampus and thalamus: a microdialysis study

Journal of Neurochemistry
Liliana Carmona AparicioMichele Simonato

Abstract

The neuropeptide nociceptin/orphanin FQ (N/OFQ) has been suggested to play a facilitatory role in kainate seizure expression. Furthermore, mRNA levels for the N/OFQ precursor are increased following kainate seizures, while its receptor (NOP) density is decreased. These data suggest increased N/OFQ release. To obtain direct evidence that this is the case, we have developed a microdialysis technique, coupled with a sensitive radioimmunoassay, that allows measurement of N/OFQ release from the hippocampus and thalamus of awake, freely moving animals. In both these brain areas, the spontaneous N/OFQ efflux decreased by approximately 50% and 65% when Ca2+ was omitted and when tetrodotoxin was added to the perfusion medium, respectively. Perfusion of the dialysis probe with high K+ increased N/OFQ release (approximately threefold) in a Ca2+-dependent and tetrodotoxin-sensitive manner. Kainate seizures caused a twofold increase in N/OFQ release followed, within 3 h, by a return to baseline levels. Approximately 5 h after kainate, a late increase in N/OFQ release was observed. On the following day, when animals were having only low grade seizures, N/OFQ release was not significantly different from normal. These phenomena were observed w...Continue Reading

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Citations

Feb 21, 2008·Journal of Molecular Neuroscience : MN·Rosalia CannarsaPatrizia Romualdi
May 19, 2009·American Journal of Respiratory Cell and Molecular Biology·Bruno D'AgostinoFrancesco Rossi
Nov 18, 2011·Journal of Pharmaceutical and Biomedical Analysis·Xingguo ZhangFanzhu Li
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Jul 1, 2010·Movement Disorders : Official Journal of the Movement Disorder Society·Matteo MartiMichele Morari
Jul 26, 2005·Peptides·Richard J Bodnar, Gad E Klein
Aug 22, 2017·Frontiers in Molecular Neuroscience·Johannes Burtscher, Christoph Schwarzer

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