PMID: 8950506Nov 1, 1996Paper

Kallistatin in human ocular tissues: reduced levels in vitreous fluids from patients with diabetic retinopathy

Current Eye Research
J X MaJ Chao

Abstract

Kallistatin is a serine proteinase inhibitor, which binds to tissue kallikrein and inhibits its proteolytic activity. This study is to determine the expression, cellular localization and the potential function of kallistatin in the eye. Tissue kallikrein-kallistatin complex formation was performed to detect the kallikrein-binding activity in ocular tissues. Immunoreactive kallistatin was detected and quantified by an enzyme-linked immunosorbent assay using polyclonal antibody specific to human kallistatin. In situ hybridization histochemistry was employed to localize the kallistatin mRNA in human eyes using an antisense riboprobe of kallistatin. We have identified active kallistatin in the cornea, ciliary body, sclera, choroid, optic nerve, retina, vitreous and aqeous fluids. Kallistatin binds to tissue kallikrein and forms an SDS-stable complex. Immunoreactive kallistatin was identified in these tissues. Linear dose-dependent curves of the tissue extracts of the retina and choroid are parallel to that of purified human kallistatin, suggesting their immunological identity. The kallistatin mRNA was identified in the ciliary muscle, lens epithelial cells, all the layers of retina cells, optic nerve, choroid and vascular endotheli...Continue Reading

References

Jan 1, 1989·Annual Review of Pharmacology and Toxicology·H S Margolius
Oct 15, 1986·The Biochemical Journal·J ChaoL Chao
Aug 1, 1984·The Journal of Clinical Endocrinology and Metabolism·R K MayfieldJ A Colwell
Apr 1, 1982·British Journal of Pharmacology·A W Cuthbert, H S Margolius
Jan 1, 1995·Annual Review of Pharmacology and Toxicology·J V Mombouli, P M Vanhoutte
Aug 1, 1994·The British Journal of Ophthalmology·R M MannersP J Fenton
Jun 1, 1994·Current Eye Research·S S TwiningX Zhou
Oct 1, 1993·The Tohoku Journal of Experimental Medicine·M YasujimaK Abe
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F R SteeleJ Tombran-Tink

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Citations

Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Bin ZhangJian-xing Ma
Oct 12, 2001·Progress in Histochemistry and Cytochemistry·M Kurpakus-WheaterL D Hazlett
Nov 1, 2006·Progress in Retinal and Eye Research·Sarah X Zhang, Jian-xing Ma
Feb 11, 2015·Reviews in Endocrine & Metabolic Disorders·Rui Cheng, Jian-xing Ma
Nov 23, 2013·Diabetes·Ling ZhaoKang Zhang
Aug 17, 2016·International Journal of Molecular Sciences·Jiaze LiJonathan Golledge
Apr 13, 2010·Progress in Retinal and Eye Research·Erica L FletcherJennifer L Wilkinson-Berka
Jul 20, 2016·Hypertension·Julie ChaoLee Chao
Jun 11, 2010·Current Diabetes Reports·Edward P Feener
Feb 10, 2007·Nature Medicine·Thomas W Gardner, David A Antonetti
Oct 18, 2002·Blood·Robert Q MiaoJulie Chao
May 1, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yanhui YangJian-Xing Ma
Oct 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H C HatcherJ X Ma
May 8, 2003·American Journal of Physiology. Cell Physiology·Robert Q MiaoJulie Chao
Jun 9, 2020·Frontiers in Immunology·Fauziyya MuhammadDarren J Lee
Dec 22, 2009·Journal of Proteome Research·Kyunggon KimYoungsoo Kim

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