Kanadaptin is a protein that interacts with the kidney but not the erythroid form of band 3.

The Journal of Biological Chemistry
J ChenQ al-Awqati

Abstract

Although epithelial membrane proteins are separately targeted to apical or basolateral domains, some are apically located in one cell type but are basolateral in others. More dramatically, the anion exchanger of a clonal cell line of intercalated cells derived from the kidney can be retargeted from the apical to basolateral domain. This Cl:HCO3 exchanger, kAE1, is an alternately spliced form of the erythroid anion exchanger (AE1, band 3), but unlike band 3 it does not bind ankyrin. Here we identify a new protein (kanadaptin) that binds to the cytoplasmic domain of kAE1 in vitro and in vivo but not to the erythroid AE1 or to ankyrin. No significant homologous proteins have been reported so far. Kanadaptin is widely expressed in epithelial (kidney, lung, and liver) and non-epithelial cells (brain and skeletal and cardiac muscle). In kidney, we found by immunocytochemistry that kanadaptin was only expressed in the collecting tubule. In the intercalated cells of this segment, it colocalized with kAE1 in cytoplasmic vesicles but not when the exchanger was in the basolateral membrane. These results raised the possibility that this protein is involved in the targeting of kAE1 vesicles to their final destination.

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Citations

Sep 16, 2000·Current Opinion in Nephrology and Hypertension·C Shayakul, S L Alper
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Emmanuelle Cordat
Feb 5, 2002·Annual Review of Physiology·Seth L Alper
Jan 1, 2012·Scientifica·Sami Dridi
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·E L PerkinsM A Resnick
Dec 23, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yoshio TakeiMakoto Kusakabe
Feb 15, 2002·Current Opinion in Hematology·Michael J A Tanner
May 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura R Saunders, Glen N Barber
Jun 8, 2007·The Journal of Biological Chemistry·Thitima KeskanokwongJoseph R Casey
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Janne A QuiltyReinhart A Reithmeier
Aug 5, 1998·The American Journal of Physiology·Q Al-AwqatiJ Takito
Mar 15, 2005·Molecular Membrane Biology·Saranya KittanakomReinhart A F Reithmeier
Jul 26, 2002·Journal of the American Society of Nephrology : JASN·Fiona E Karet

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