KapB is a lipoprotein required for KinB signal transduction and activation of the phosphorelay to sporulation in Bacillus subtilis

Molecular Microbiology
V DartoisJames A Hoch

Abstract

KinB is one of the two major histidine kinases that provide phosphate input in the phosphorelay to produce SpoOA approximately P, the key transcription factor controlling the initiation of sporulation. A search for insertion mutants affected in activation of KinB-dependent sporulation led to the identification of the lgt locus encoding the lipoprotein glyceryltransferase required for the lipid modification of prolipoproteins before their cleavage and translocation across the cytoplasmic membrane. In parallel, a putative lipoprotein signal peptide cleavage site was detected in KapB, known to be strictly required for KinB-mediated sporulation and located downstream of KinB in a single transcription unit. Using PhoA peptide fusions, we have shown that KapB signal-peptide can direct active alkaline phosphatase to the outer surface of the cytoplasmic membrane in an LGT-dependent manner, strongly suggesting that KapB is a lipoprotein tethered to the outer face of the cytoplasmic membrane via a lipid anchor. As KapB proved to be dispensable for expression of the kinBkapB operon, a chimeric kinase was built consisting of KinA sensor domain fused to KinB kinase domain (KinA'-'B) to assess (i) the involvement of KapB in catalysis of the ...Continue Reading

Citations

Nov 26, 2010·Journal of Bacteriology·Anna L McLoonRichard Losick
Feb 20, 2007·Journal of Bacteriology·Hendrik SzurmantJames A Hoch
Jan 22, 2013·Journal of Bacteriology·Sharon Garti-LeviSigal Ben-Yehuda
May 6, 2004·Journal of Bacteriology·Takao IgarashiPeter Setlow
Jul 21, 2005·Journal of Bacteriology·Hendrik SzurmantJames A Hoch
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Mitsuo OguraTeruo Tanaka
Dec 9, 2008·Trends in Microbiology·Matthew I HutchingsIain C Sutcliffe
Apr 29, 2014·Journal of Basic Microbiology·Eisha MhatreAkos T Kovács
Aug 29, 2006·Trends in Microbiology·Paul A Hoskisson, Matthew I Hutchings
Dec 25, 2009·FEMS Microbiology Reviews·Daniel López, Roberto Kolter
Nov 13, 2004·FEMS Microbiology Reviews·Iain C Sutcliffe, Dean J Harrington
Jul 30, 2014·PloS One·Adrian D LandPetra Anne Levin
Feb 4, 2012·PLoS Biology·Joe H LevineMichael B Elowitz
Nov 13, 2008·Structure·Daniel M SullivanJohn Cavanagh
Feb 24, 2009·Journal of Dairy Science·M A Azcarate-PerilT R Klaenhammer
Mar 4, 2006·Microbiology·Claudia S LópezCarmen Sánchez-Rivas
Aug 28, 2020·Frontiers in Microbiology·I-Chiao LeeMichiel Kleerebezem
Jan 12, 1999·Journal of Bacteriology·J BengtssonL Hederstedt

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