Kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen modulates K1 expression through its cis-acting elements within the terminal repeats

Journal of Virology
Subhash C VermaE S Robertson

Abstract

K1 is the first open reading frame encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) and lies positionally to the immediate right of the terminal repeats. K1 is a transmembrane glycoprotein having a functional immunoreceptor tyrosine-based activation motif (ITAM) capable of activating B-cell receptor signaling. K1 is expressed mostly during the lytic cycle of the virus and its promoter lies within the terminal repeat which contains the binding sites for latency-associated nuclear antigen (LANA). The K1 promoter (K1p) having LANA binding sites assayed by reporter assay demonstrated that LANA is capable of down-regulating K1 promoter transcriptional activity. However, the KSHV replication transcription activator RTA up-regulates K1p transcriptional activity. The promoter deleted of LANA binding sites showed loss in LANA-mediated down-regulation but was unaffected for RTA-mediated up-regulation. Increasing amounts of RTA rescued LANA-mediated repression of K1p transcriptional activity in cotransfection experiments. Reporter assay data suggest that LANA binding to its cognate sequence is critical for LANA-mediated repression of K1p as a LANA construct lacking the DNA binding domain was unable to repress K1p transcription. A...Continue Reading

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Citations

Nov 21, 2008·Journal of Virology·Sebastien ApcherRobin Fåhraeus
Aug 24, 2012·Journal of Virology·Aline C HabisonKenneth M Kaye
Mar 25, 2010·PLoS Pathogens·Grzegorz SarekPäivi M Ojala
Jun 10, 2010·PLoS Pathogens·Thomas Günther, Adam Grundhoff
Jun 4, 2016·International Journal of Molecular Sciences·Enea Gino Di DomenicoFabrizio Ensoli
Oct 13, 2014·Molecular Oncology·Richard K DzengErle S Robertson
Jul 21, 2015·Reviews in Medical Virology·Annie Cristhine Moraes Sousa-SquiavinatoDeilson Elgui De Oliveira
Aug 28, 2021·Biomolecules·Nieves Lara-Ureña, Mario García-Domínguez
Apr 17, 2007·Molecular Cancer·György StuberLaszlo Szekely

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