Kaposi's sarcoma-associated herpesvirus ORF57 functions as a viral splicing factor and promotes expression of intron-containing viral lytic genes in spliceosome-mediated RNA splicing

Journal of Virology
Vladimir MajerciakZhi-Ming Zheng

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 facilitates the expression of both intronless viral ORF59 genes and intron-containing viral K8 and K8.1 genes (V. Majerciak, N. Pripuzova, J. P. McCoy, S. J. Gao, and Z. M. Zheng, J. Virol. 81:1062-1071, 2007). In this study, we showed that disruption of ORF57 in a KSHV genome led to increased accumulation of ORF50 and K8 pre-mRNAs and reduced expression of ORF50 and K-bZIP proteins but had no effect on latency-associated nuclear antigen (LANA). Cotransfection of ORF57 and K8beta cDNA, which retains a suboptimal intron of K8 pre-mRNA due to alternative splicing, promoted RNA splicing of K8beta and production of K8alpha (K-bZIP). Although Epstein-Barr virus EB2, a closely related homolog of ORF57, had a similar activity in the cotransfection assays, herpes simplex virus type 1 ICP27 was inactive. This enhancement of RNA splicing by ORF57 correlates with the intact N-terminal nuclear localization signal motifs of ORF57 and takes place in the absence of other viral proteins. In activated KSHV-infected B cells, KSHV ORF57 partially colocalizes with splicing factors in nuclear speckles and assembles into spliceosomal complexes in association with low-abundance viral ORF50 and K8 p...Continue Reading

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Citations

Sep 21, 2012·Journal of Virology·Guy R PilkingtonBarbara K Felber
Jun 1, 2011·Advances in Virology·Fengchun YeShou-Jiang Gao
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Vladimir Majerciak, Zhi-Ming Zheng
Feb 13, 2015·Viruses·Vladimir Majerciak, Zhi-Ming Zheng
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Nov 18, 2021·Journal of Virology·Beatriz Alvarado-HernandezZhi-Ming Zheng

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