kappa-Conotoxin PVIIA is a peptide inhibiting the shaker K+ channel.

The Journal of Biological Chemistry
K J ShonBaldomero M Olivera

Abstract

kappa-Conotoxin PVIIA (kappa-PVIIA), a 27-amino acid toxin from Conus purpurascens venom that inhibits the Shaker potassium channel, was chemically synthesized in a biologically active form. The disulfide connectivity of the peptide was determined. kappa-Conotoxin PVIIA has the following structure. This is the first Conus peptide known to target K+ channels. [structure: see text] Although the Shaker K+ channel is sensitive to kappa-PVIIA, the rat brain Kv1.1 subtype is resistant. Chimeras between Shaker and the Kv1.1 K+ channels were constructed and expressed in Xenopus oocytes. Only channels containing the putative pore-forming region between the fifth and sixth transmembrane domains of Shaker retained toxin sensitivity, indicating that the toxin target site is in this region of the channel. Evidence is presented that kappa-PVIIA interacts with the external tetraethyl-ammonium binding site on the Shaker channel. Although both kappa-PVIIA and charybdotoxin inhibit the Shaker channel, they must interact differently. The F425G Shaker mutation increases charybdotoxin affinity by 3 orders of magnitude but abolishes kappa-PVIIA sensitivity. The precursor sequence of kappa-PVIIA was deduced from a cDNA clone, revealing a prepropeptid...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·W Stühmer
Sep 22, 1995·The Journal of Biological Chemistry·C HopkinsB M Olivera
Jan 1, 1994·Annual Review of Biochemistry·B M OliveraM E Adams
Nov 1, 1993·Neuropharmacology·V D MonjeW R Gray
Oct 1, 1993·Protein Science : a Publication of the Protein Society·W R Gray

❮ Previous
Next ❯

Citations

May 24, 2000·Journal of Molecular Recognition : JMR·K J NielsenR Lewis
Oct 25, 2002·Diabetes/metabolism Research and Reviews·Silvia Mora, Jeffrey E Pessin
Dec 7, 2002·Diabetes/metabolism Research and Reviews·Susanne G Straub, Geoffrey W G Sharp
Aug 26, 2009·Cellular and Molecular Life Sciences : CMLS·Reed B Jacob, Owen M McDougal
Mar 15, 2008·Applied Microbiology and Biotechnology·Stefan Becker, Heinrich Terlau
Aug 2, 2000·Biophysical Journal·V AvdoninT Hoshi
Aug 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·B M Olivera, L J Cruz
Oct 16, 2001·Toxicon : Official Journal of the International Society on Toxinology·D J EspirituB M Olivera
Jun 22, 2002·Toxicon : Official Journal of the International Society on Toxinology·M LirazanL J Cruz
Aug 2, 2003·Toxicon : Official Journal of the International Society on Toxinology·Silke KaufersteinDietrich Mebs
Feb 17, 2000·Drug Discovery Today·G S ShenR T McCabe
Aug 19, 2007·Biochemistry·Olga BuczekRaymond S Norton
Feb 2, 2008·Nature Reviews. Drug Discovery·Tisha Joy, Robert A Hegele
May 29, 2008·The Journal of Biological Chemistry·Julita S ImperialBaldomero M Olivera
Jul 10, 1999·The Journal of General Physiology·H TerlauF Conti
Aug 13, 2004·Proceedings. Biological Sciences·Thomas F Duda, Stephen R Palumbi
Sep 9, 2006·Journal of Gastroenterology and Hepatology·Scott L Friedman
Jun 21, 2013·Marine Drugs·Jorge NevesVitor Vasconcelos
Mar 26, 2013·Cellular and Molecular Life Sciences : CMLS·Jennifer J SmithPaul F Alewood
Feb 16, 2016·Biochemistry. Biokhimii︠a︡·A I KuzmenkovA A Vassilevski
Oct 18, 2006·The FEBS Journal·Yu-Hong HanCheng-Wu Chi
Jun 7, 2015·Analytical and Bioanalytical Chemistry·Alena M RodriguezFrank Marí
Oct 28, 2008·Toxicon : Official Journal of the International Society on Toxinology·Mandë HolfordBaldomero M Olivera
Jun 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Muna AffaraCristin Print
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Laszlo Tretter, Vera Adam-Vizi
Nov 17, 2012·Open Biology·Mikkel A AlgireChuck Merryman
Dec 15, 2006·Peptides·Karen S Luna-RamírezMaría Maillo
Dec 8, 2017·Toxins·Bingmiao GaoQiong Shi
Nov 26, 2003·Journal of Cardiovascular Pharmacology·Shi Jun ZhangJames M Downey
Feb 1, 2003·FEBS Letters·Alessandro Grottesi, Mark S P Sansom
Nov 16, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sulan LuoYuanyan Hu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.