Kappa opioid receptor activation of p38 MAPK is GRK3- and arrestin-dependent in neurons and astrocytes.

The Journal of Biological Chemistry
Michael R BruchasCharles Chavkin

Abstract

AtT-20 cells expressing the wild-type kappa opioid receptor (KOR) increased phospho-p38 MAPK following treatment with the kappa agonist U50,488. The increase was blocked by the kappa antagonist norbinaltorphimine and not evident in untransfected cells. In contrast, U50,488 treatment of AtT-20 cells expressing KOR having alanine substituted for serine-369 (KSA) did not increase phospho-p38. Phosphorylation of serine 369 in the KOR carboxyl terminus by G-protein receptor kinase 3 (GRK3) was previously shown to be required for receptor desensitization, and the results suggest that p38 MAPK activation by KOR may require arrestin recruitment. This hypothesis was tested by transfecting arrestin3-(R170E), a dominant positive form of arrestin that does not require receptor phosphorylation for activation. AtT-20 cells expressing both KSA and arrestin3-(R170E) responded to U50,488 treatment with an increase in phospho-p38 consistent with the hypothesis. Primary cultured astrocytes (glial fibrillary acidic protein-positive) and neurons (gamma-aminobutyric acid-positive) isolated from mouse striata also responded to U50,488 by increasing phospho-p38 immunolabeling. p38 activation was not evident in either striatal astrocytes or neurons iso...Continue Reading

References

Nov 1, 1996·Neurochemical Research·E T PirosC J Evans
Feb 1, 1997·Journal of Neuroscience Methods·G J Brewer
Sep 16, 1999·Cellular and Molecular Life Sciences : CMLS·L A Tibbles, J R Woodgett
Mar 4, 2000·Bioorganic & Medicinal Chemistry Letters·K LackeyE R Wood
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A MócsaiE Ligeti
Feb 7, 2001·Science·E M UllianB A Barres
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L M LuttrellR J Lefkowitz
Jun 30, 2001·Trends in Pharmacological Sciences·M J Marinissen, J S Gutkind
Oct 5, 2001·Nature Reviews. Neuroscience·K L Pierce, R J Lefkowitz
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·J S Gutkind
Jan 10, 2002·The Journal of Biological Chemistry·Jeremy CelverVsevolod V Gurevich
Jun 20, 2003·The Journal of Biological Chemistry·Jay P McLaughlinCharles Chavkin
Sep 3, 2003·Nature Reviews. Drug Discovery·Sanjay KumarJohn C Lee
Feb 21, 2004·Nature Reviews. Neuroscience·Gareth M Thomas, Richard L Huganir
May 14, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mei XuCharles Chavkin
Jan 7, 2005·Molecular Pharmacology·Dacia L HuntonRobert J Lefkowitz
Apr 23, 2005·Science·Robert J Lefkowitz, Sudha K Shenoy
Jun 10, 2005·The Journal of Biological Chemistry·Mariana M BelchevaCarmine J Coscia
Oct 8, 2005·Science·Olivier PascualPhilip G Haydon

❮ Previous
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Citations

Apr 20, 2010·Psychopharmacology·Michael R Bruchas, Charles Chavkin
Jul 10, 2013·Psychopharmacology·Ashlee Van't Veer, William A Carlezon
Jul 13, 2013·Cellular and Molecular Neurobiology·Feng LiDian-Shuai Gao
Jul 8, 2011·Biochemistry·Sergio CoffaVsevolod V Gurevich
Dec 7, 2013·ACS Chemical Neuroscience·Ellen PhamduongCarmine J Coscia
Jan 31, 2013·Journal of Medicinal Chemistry·F Ivy Carroll, William A Carlezon
Aug 24, 2010·Acta Pharmacologica Sinica·Yu-hua WangJing-gen Liu
Jun 22, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ream Al-HasaniMichael R Bruchas
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jane E MurphyNigel W Bunnett
Oct 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Benjamin B LandCharles Chavkin
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nien-Pei TsaiLi-Na Wei
Jun 28, 2012·The Journal of Biological Chemistry·Marie-Laure RivesJonathan A Javitch
Sep 24, 2009·The Journal of Biological Chemistry·Cecilea C ClaytonCharles Chavkin
Jan 26, 2012·The Journal of Biological Chemistry·Luis E GimenezVsevolod V Gurevich
Apr 24, 2012·The Journal of Biological Chemistry·Maya BreitmanVsevolod V Gurevich
Oct 23, 2012·The Journal of Biological Chemistry·Selena S SchattauerCharles Chavkin
Nov 13, 2008·The Journal of Biological Chemistry·Xiufeng SongVsevolod V Gurevich
Dec 14, 2011·American Journal of Physiology. Cell Physiology·Gavin E MorrisJonathon M Willets
Jan 15, 2013·Molecular Endocrinology·Diane Gesty-PalmerStuart Maudsley
Sep 8, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Julia C LemosCharles Chavkin
Dec 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Abigail G SchindlerCharles Chavkin
Mar 9, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mei XuCharles Chavkin
Oct 26, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael R BruchasCharles Chavkin
Jan 11, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benjamin B LandCharles Chavkin
Jan 22, 2014·Behavioural Brain Research·Cindee F RoblesBrian C Trainor
Oct 18, 2011·Cellular and Molecular Life Sciences : CMLS·H A TejedaR Henriksson
Feb 13, 2016·Scientific Reports·Xuanzhi ZhanVsevolod V Gurevich
Aug 24, 2013·Journal of Neuroimmunology·Christine BörnerJürgen Kraus
Aug 26, 2015·Neuropharmacology·Emeline L MailletDeborah C Mash
Jun 6, 2012·Cellular Signalling·Vsevolod V Gurevich, Eugenia V Gurevich
Sep 10, 2011·Pharmacology & Therapeutics·Eugenia V GurevichVsevolod V Gurevich
Dec 25, 2010·Trends in Molecular Medicine·Erin J WhalenRobert J Lefkowitz
Jun 2, 2009·Pharmacology & Therapeutics·Christoph Schwarzer
Nov 27, 2014·Journal of Medicinal Chemistry·Andrew P RileyThomas E Prisinzano

❮ Previous
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