Karmitoxin production by Karlodinium armiger and the effects of K. armiger and karmitoxin towards fish.

Harmful Algae
Sofie Bjørnholt BinzerPer Juel Hansen

Abstract

The dinoflagellate Karlodinium armiger has a huge impact on wild and caged fish during blooms in coastal waters. Recently, a new toxin, karmitoxin, was chemically characterized from K. armiger and a quantification method was established, thereby allowing investigations of the fish killing mechanism. K. armiger is not able to grow in standard growth media that are based on nitrate as a nitrogen source, and successful cultures of this species have only been achieved in mixotrophic cultures after addition of a prey source. Here we show that addition of ammonium (up to 50 µM) to the growth media is a good alternative, as K. armiger batch cultures achieve growth rates, which are comparable to growth rates reached in mixotrophic cultures. Karmitoxin production (1.9 and 2.9 pg cell-1 d-1) and cellular karmitoxin content (8.72 ± 0.25 pg cell-1 and 7.14 ± 0.29 pg cell-1) were in the same range, though significantly different, in prey-fed cultures and monocultures supplied with ammonium, respectively. Net production of karmitoxin stopped when the K. armiger cultures reached stationary growth phase, indicating no accumulation of karmitoxin in cells or growth media. Toxicity tests towards sheepshead minnow fish larvae indicated rapid death...Continue Reading

References

Nov 9, 2004·Toxicon : Official Journal of the International Society on Toxinology·Lincoln MacKenzieAndy Selwood
Feb 23, 2008·Biochimica Et Biophysica Acta·Nagy MorsySaburo Aimoto
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jian ShengAllen R Place
Feb 17, 2010·Journal of the American Chemical Society·Jiangnan PengMark T Hamann
Aug 28, 2010·Journal of Natural Products·Ryan M Van WagonerJeffrey L C Wright
Nov 3, 2008·Tetrahedron Letters·Ryan M Van WagonerJeffrey L C Wright
Sep 23, 2010·Toxicon : Official Journal of the International Society on Toxinology·Chengxu ZhouXiaojun Yan
Apr 20, 2012·The ISME Journal·Terje BergePer Juel Hansen
Dec 30, 2014·Aquatic Toxicology·Jonathan R DeedsJoseph P Y Kao
Dec 31, 2015·PloS One·Christian RitzDaniel Gerhard
Apr 6, 2017·Journal of Natural Products·Silas Anselm RasmussenThomas Ostenfeld Larsen
Sep 1, 2017·Marine Drugs·Aaron John Christian AndersenThomas Ostenfeld Larsen

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