Kartogenin inhibits pain behavior, chondrocyte inflammation, and attenuates osteoarthritis progression in mice through induction of IL-10

Scientific Reports
Ji Ye KwonMi La Cho

Abstract

Osteoarthritis (OA) is a major degenerative joint condition that causes articular cartilage destruction. It was recently found that enhancement of chondroclasts and suppression in Treg cell differentiation are involved in the pathogenesis of OA. Kartogenin (KGN) is a small drug-like molecule that induces chondrogenesis in mesenchymal stem cells (MSCs). This study aimed to identify whether KGN can enhance severe pain behavior and improve cartilage repair in OA rat model. Induction of OA model was loaded by IA-injection of MIA. In the OA rat model, treatment an intra-articular injection of KGN. Pain levels were evaluated by analyzing PWL and PWT response in animals. Histological analysis and micro-CT images of femurs were used to analyze cartilage destruction. Gene expression was measured by real-time PCR. Immunohistochemistry was analyzed to detect protein expression. KGN injection significantly decreased pain severity and joint destruction in the MIA-induced OA model. KGN also increased mRNA levels of the anti-inflammatory cytokine IL-10 in OA patients' chondrocytes stimulated by IL-1β. Decreased chondroclast expression, and increased Treg cell expression. KGN revealed therapeutic activity with the potential to reduce pain and ...Continue Reading

References

Dec 7, 2000·Cytokines, Cellular & Molecular Therapy·S KanekoJ Kagawa
Mar 30, 2001·The Journal of Biological Chemistry·M KashiwagiK Brew
Sep 9, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Peter S BurrageConstance E Brinckerhoff
Dec 2, 2010·Nature Reviews. Rheumatology·Mohit KapoorHassan Fahmi
Jul 8, 2011·PM & R : the Journal of Injury, Function, and Rehabilitation·Michael B Mueller, Rocky S Tuan
Apr 12, 2012·Science·Kristen JohnsonPeter G Schultz
Jun 8, 2012·Neuromodulation : Journal of the International Neuromodulation Society·Erin D MilliganMelissa J Mahoney
Dec 1, 2012·Osteoarthritis and Cartilage·F Berenbaum
Nov 1, 2013·Tissue Engineering. Part B, Reviews·Dominick J Blasioli, David L Kaplan
May 31, 2014·Mediators of Inflammation·Piotr WojdasiewiczDariusz Szukiewicz
Apr 6, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shufeng LiWendy Smith
Apr 21, 2017·Frontiers in Immunology·Yu-Sheng LiGuang-Hua Lei

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Citations

Nov 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hae Lim KimSeung Hwan Yang
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Yanhong ZhaoYanmei Zhao
Nov 12, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shuyi Xiao, Liang Chen

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Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
PCR

Software Mentioned

GraphPad Prism
NRecon

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