PMID: 6169427Aug 1, 1981Paper

Karyotyping chromosomes by electron microscopy. Condensation-inhibition of G bands in human and Chinese hamster chromosomes by a BrdU-Hoechst 33258 treatment

Cancer Genetics and Cytogenetics
V J Goyanes, J Mendez

Abstract

A BrdU-Hoechst 33258 treatment of living cells, which selectively induced condensation-inhibition of G-band chromatin in human and Chinese hamster chromosomes, is presented. As a consequence mitotic chromosomes showed high resolution R-banding patterns when examined by light and electron microscopy. Besides each whole chromosome identification, this procedure also permitted the electron microscopic study of specific structures, such as satellites, secondary constrictions, telomeres, centromeres, as well as G and R bands, some of them no visible by light microscopy. We have also observed that the chromatin of G and R bands behave as blocks of chromatin condensation and that G-band chromatin develops condensation along G2. Under the BrdU-Hoechst 33258 treatment, chromatin fibers seem to invert their spontaneous pattern of condensation within the chromosomes.

References

Jan 1, 1975·Cytogenetics and Cell Genetics·J L Hamerton
Jun 30, 1976·Chromosoma·A RocchiM Di Castro
Jan 1, 1976·Cytogenetics and Cell Genetics·S PimpinelliM Gatti
May 1, 1978·Experimental Cell Research·S Matsukuma, T Utakoji
Aug 1, 1979·Experimental Cell Research·J J Yunis, G F Bahr
Mar 26, 1976·Science·J J Yunis

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Citations

Feb 15, 1985·Cancer Genetics and Cytogenetics·V J Goyanes
Mar 9, 2005·Cytogenetic and Genome Research·E Costas, V Goyanes
Jan 1, 1990·American Journal of Medical Genetics. Supplement·A Serra, R Bova
Jan 1, 1988·Genetica·A MartínezJ Méndez

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