Kat1-antigen--a reliable immunological marker for identifying osteoclast precursors of rats: detection of subpopulations among precursors and initiation of osteoclastogenesis

Histochemistry and Cell Biology
T KukitaT Iijima

Abstract

Previously we found a unique cell surface antigen [Kat1-antigen (Kat1-Ag)] expressed on rat osteoclasts. In the present study, we focused on the expression of this antigen in preosteoclasts, mononuclear precursors of osteoclasts. Immunohistochemical and immunoelectron microscopic observations of the Kat1-Ag expressed in vivo showed the antigen to be present on mononuclear cells having the morphological features of preosteoclasts. The relationship between Kat1-Ag expression and calcitonin receptor (CTR) expression was examined in detail by a double-detection technique for CTR and Kat1-Ag by use of autoradiography and immunocytochemistry, respectively. In a culture system for forming mononuclear preosteoclast-like cells, almost 100% of the mononuclear cells expressing Kat1-Ag also expressed CTR, demonstrating that Kat1-Ag is a reliable immunological marker for identifying preosteoclasts. Interestingly, a significant number of the CTR-positive mononuclear cells did not express the Kat1-Ag. Detection of these cells expressing CTR but not Kat1-Ag strongly suggests the presence of subpopulations in preosteoclasts. We also obtained evidence suggesting that expression of the Kat1-Ag is initiated during the postmitotic stage of the oste...Continue Reading

Citations

Nov 7, 2012·Journal of Cellular Biochemistry·Akira TakahashiToshio Kukita
Dec 17, 2002·Histochemistry and Cell Biology·Esther Asan
Oct 10, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yin-Ji LiToshio Kukita
Oct 7, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Toshio KukitaAkiko Kukita

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