KAT45, a noradrenergic human pheochromocytoma cell line producing corticotropin-releasing hormone

Endocrinology
M VenihakiA N Margioris

Abstract

KAT45 cells were derived from a human pheochromocytoma, which also caused ectopic Cushing's syndrome, and developed into a cell line spontaneously after the continuous primary culture of the tumor cells. These human pheochromocytoma cells were compared with the extensively characterized PC12 rat pheochromocytoma cell line. KAT45 cells resembled PC12 cells in morphology, proliferation rate, response to cholinergic stimuli, and the development of dendrite-like projections after exposure to nerve growth factor. They produced norepinephrine and epinephrine in a ratio of 50:1, as opposed to production of dopamine by PC12 cells, in amounts 1 order of magnitude higher compared with PC12. Because of the ectopic Cushing's syndrome in our patient, her normal ACTH level, and the knowledge that PC12 cells and even normal rat chromaffin cells appear to produce CRH, we examined whether KAT45 cells also produced this neuropeptide. Indeed, KAT45 cells released authentic CRH and contained an apparently intact CRH transcript. Nicotine and KCl depolarization stimulated the secretion of CRH, whereas interleukin-1beta, glucocorticoids, and nerve growth factor stimulated its synthesis. In addition to the potential systemic effects of CRH, which in o...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Nov 1, 1992·Annals of Clinical Biochemistry·A HatzoglouE Castanas
May 1, 1991·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·M SchallingT Hökfelt
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K AlheimT Bartfai
May 1, 1989·Journal of Molecular Endocrinology·Y de KeyzerX Bertagna
Jan 1, 1989·Neuroscience·M SchultzbergT Bartfai
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B G RobinsonJ A Majzoub
Jun 10, 1988·European Journal of Pharmacology·M C Olianas, P Onali
Nov 1, 1984·The Journal of Clinical Endocrinology and Metabolism·T SudaK Shizume
May 18, 1995·The New England Journal of Medicine·G P Chrousos
Nov 1, 1994·Biological Psychiatry·J A PosenerA F Schatzberg
Nov 1, 1994·In Vitro Cellular & Developmental Biology. Animal·M F Melzig
May 1, 1994·Neuroscience Research·E H LeeA Y Chen
Jan 14, 1994·Biochemical and Biophysical Research Communications·N YanagiharaF Izumi

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Citations

Mar 1, 2012·World Journal of Surgery·James Kirkby-BottBruno Carnaille
Feb 6, 2010·European Journal of Pharmacology·Li-Zhen YangJoachim Spiess
Dec 29, 2006·Annals of the New York Academy of Sciences·Evangelia Zapanti, Ioannis Ilias
May 26, 2005·The Journal of Clinical Endocrinology and Metabolism·Tsuyoshi FukudaHironobu Sasano
Jul 5, 2005·Expert Opinion on Investigational Drugs·D Gurwitz

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