KATP channel subunits are expressed in the epididymal epithelium in several mammalian species

Biology of Reproduction
Pascale LybaertPhilippe Lebrun

Abstract

Adenosine triphosphate-sensitive K(++) (K(ATP)) channels are poorly characterized in the reproductive tract. The present study was designed to evaluate the putative expression of K(ATP) channel subunits (Kir6.x and SURx) in the epididymis from different mammalian species. Immunohistochemical, Western blot, and RT-PCR techniques were used. A positive immunostaining for Kir6.2 (KCNJ11) and SUR2 (ABCC9) was observed by immunoenzymatic and immunofluorescent approaches in the principal epithelial cells throughout all regions of the rat and mouse epididymis. Double labeling with anti-aquaporin 9 (AQP9) and anti-Kir6.2 (KCNJ11) confirmed their colocalization in the principal cells. No immunostaining could be demonstrated for Kir6.1 (KCNJ8) and SUR1 (ABCC8) subunits. Under higher magnification, the immunostaining for Kir6.2 (KCNJ11) exhibited a cytoplasmic labeling that was more intense at the level of the Golgi apparatus along the whole epididymis. A similar pattern was observed for SUR2 (ABCC9), although in the latter case, the Golgi labeling appeared to be region specific. Spermatozoa in epididymal tubules from rodents also immunostained for Kir6.2 (KCNJ11) and SUR2 (ABCC9). Western blot analysis of epididymal total protein and crud...Continue Reading

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Citations

Jan 28, 2010·Molecular Pain·Vasiliki ZogaConstantine Sarantopoulos
Oct 11, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·R S TavaresS Amaral
Nov 2, 2018·Current Medicinal Chemistry·R S TavaresS Amaral
Jan 23, 2019·Frontiers in Physiology·Dong-Dong GaoWen-Liang Zhou
Oct 30, 2021·Journal of Neuroscience Research·Enes AkyuzYam Nath Paudel

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Methods Mentioned

BETA
biopsies
Protein Extraction
Protein Assay
electrophoresis
PCR

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