Kbtbd5 is regulated by MyoD and restricted to the myogenic lineage

Differentiation; Research in Biological Diversity
Kathy M BowlinXiaozhong Shi

Abstract

BTB-BACK-Kelch (BBK) proteins play broad roles in cellular and molecular regulation. The role of BBK proteins in the skeletal muscle lineage and myogenesis remains an active area of research. Herein, we report a novel BBK gene, Kbtbd5, which we discovered and found to be restricted to the myogenic lineage. We observed that Kbtbd5 was absent in proliferating myoblasts and upregulated upon myogenic differentiation. In situ hybridization analysis revealed that Kbtbd5 was restricted to the skeletal muscle lineage during embryogenesis. We identified a conserved 1.2kb upstream region, which directs reporter expression to the developing skeletal muscle lineage. Transcriptional and mutagenesis assays demonstrated that the E-box motifs contribute to the Kbtbd5 promoter activity. We have also demonstrated the in vivo and in vitro binding between MRFs and the E-box motif in the 1.2kb promoter of the Kbtbd5 gene. Our studies have revealed that the Myod family can transactivate the 1.2kb-luc reporter through the E-box motifs. In addition, we have shown that Kbtbd5 can recruit the Cullin 3 complex in vivo. Using shRNA knockdown, our study has revealed that Kbtbd5 plays an important role in the myogenic differentiation. In summary, we have de...Continue Reading

References

Dec 6, 1997·Current Opinion in Genetics & Development·K Walsh, H Perlman
Nov 6, 1998·Nature·D J GarryR S Williams
Dec 22, 1999·Trends in Cell Biology·J AdamsL Cooley
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Mary Elizabeth PownallCharles P Emerson
Jul 3, 2003·Nature Reviews. Genetics·Maura H ParkerMichael A Rudnicki
Oct 7, 2003·Nature Cell Biology·Manabu FurukawaYue Xiong
Jan 24, 2004·Current Biology : CB·Sander van den Heuvel
Jun 25, 2004·Nucleic Acids Research·Gabriela G Loots, Ivan Ovcharenko
Nov 9, 2004·The Journal of Biological Chemistry·Lionel A TintignacSerge A Leibovitch
Nov 17, 2004·Trends in Biochemical Sciences·Peter J Stogios, Gilbert G Privé
Feb 12, 2005·Genes & Development·Alexandre BlaisBrian David Dynlacht
Aug 16, 2005·Seminars in Cell & Developmental Biology·Charlotte A Berkes, Stephen J Tapscott
Jul 5, 2006·Genes & Development·Xiaozhong Shi, Daniel J Garry
Nov 23, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Roberto Perez-TorradoPierre-Antoine Defossez
Mar 17, 2007·The EMBO Journal·Annette P MeesonDaniel J Garry
Mar 21, 2008·The EMBO Journal·Julie Lagirand-CantaloubeSerge A Leibovitch
Aug 1, 2009·Mechanisms of Development·Alaa Abou-ElhamdAndrea Münsterberg
Nov 17, 2009·Developmental Biology·Matthew S AlexanderDaniel J Garry
Mar 24, 2010·The Biochemical Journal·Xiaozhong Shi, Daniel J Garry
Nov 11, 2010·Annals of the New York Academy of Sciences·David Glass, Ronenn Roubenoff
Mar 4, 2011·American Journal of Physiology. Cell Physiology·Camille W PaxtonJennifer M Pell
Apr 6, 2012·Molecular and Cellular Biochemistry·Xiaozhong Shi, Daniel J Garry
Jun 22, 2012·The Biochemical Journal·Xiaozhong ShiDaniel J Garry

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Citations

Jun 25, 2014·Skeletal Muscle·Vandana A Gupta, Alan H Beggs
Oct 17, 2017·F1000Research·Chris D BalakLisa Baumbach-Reardon
Jul 31, 2019·Journal of Neuropathology and Experimental Neurology·Han-Chih Hencher LeeChloe Miu Mak
Jan 28, 2020·Molecular Genetics & Genomic Medicine·Sai Suma K SamudralaAoy Tomita-Mitchell
Oct 28, 2019·PloS One·Hakan Claes RundqvistEva Jansson
Sep 24, 2015·The Journal of Biological Chemistry·Naoko Koyano-NakagawaDaniel J Garry
Oct 30, 2020·International Journal of Molecular Sciences·Jordan BlondelleStephan Lange
Nov 14, 2020·International Journal of Molecular Sciences·Kenneth C EhrlichMelanie Ehrlich
May 3, 2021·Biochemical and Biophysical Research Communications·Genghua ChenWen Luo

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