KCC2 Regulates Dendritic Spine Formation in a Brain-Region Specific and BDNF Dependent Manner

Cerebral Cortex
Patricia N AwadGraziella Di Cristo

Abstract

KCC2 is the major chloride extruder in neurons. The spatiotemporal regulation of KCC2 expression orchestrates the developmental shift towards inhibitory GABAergic drive and the formation of glutamatergic synapses. Whether KCC2's role in synapse formation is similar in different brain regions is unknown. First, we found that KCC2 subcellular localization, but not overall KCC2 expression levels, differed between cortex and hippocampus during the first postnatal week. We performed site-specific in utero electroporation of KCC2 cDNA to target either hippocampal CA1 or somatosensory cortical pyramidal neurons. We found that a premature expression of KCC2 significantly decreased spine density in CA1 neurons, while it had the opposite effect in cortical neurons. These effects were cell autonomous, because single-cell biolistic overexpression of KCC2 in hippocampal and cortical organotypic cultures also induced a reduction and an increase of dendritic spine density, respectively. In addition, we found that the effects of its premature expression on spine density were dependent on BDNF levels. Finally, we showed that the effects of KCC2 on dendritic spine were dependent on its chloride transporter function in the hippocampus, contrary t...Continue Reading

References

Sep 1, 1989·The Journal of Physiology·Y Ben-AriJ L Gaiarsa
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·M KorteT Bonhoeffer
Apr 16, 2003·The Journal of Cell Biology·Huaye ZhangAlan F Horwitz
Sep 23, 2003·The Journal of Physiology·William J Tyler, Lucas Pozzo-Miller
May 14, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Claudio RiveraKai Kaila
Oct 29, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bidisha ChattopadhyayaZ Josh Huang
Jun 4, 2005·The European Journal of Neuroscience·Hanmi LeeKarl Kandler
Jun 18, 2005·The Journal of Physiology·Ilona ChudotvorovaIgor Medina
Oct 13, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Peter BlaesseHans Gerd Nothwang
Jan 20, 2007·Current Opinion in Neurobiology·Hubert Fiumelli, Melanie A Woodin
May 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Laura CanceddaMu-ming Poo
Jun 6, 2008·Epilepsy Research·Aristea S Galanopoulou
Apr 22, 2009·The Journal of Cell Biology·Pirta HotulainenPekka Lappalainen
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yang ShiIryna M Ethell
Sep 10, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stanislav KhirugPeter Blaesse
Oct 1, 2010·The European Journal of Neuroscience·Rita BáldiGábor Tamás
Jan 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anastasia LudwigClaudio Rivera
Sep 1, 2011·Proceedings of the National Academy of Sciences of the United States of America·Grégory GauvainJean Christophe Poncer
Oct 4, 2011·Current Opinion in Neurobiology·Miquel Bosch, Yasunori Hayashi
Nov 9, 2011·Frontiers in Cellular Neuroscience·Ilgam KhalilovIgor Medina
Jul 19, 2012·Nature Communications·Marco dal MaschioLaura Cancedda
Sep 27, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ingrid ChammaSabine Lévi
Oct 4, 2013·Neural Plasticity·Francesco Ferrini, Yves De Koninck
Mar 19, 2014·The Journal of Comparative Neurology·Marika MarkkanenMatti S Airaksinen
Mar 22, 2014·Current Opinion in Neurobiology·Kai KailaMartin Puskarjov
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Roberto ArayaRafael Yuste
Sep 23, 2014·Nature Reviews. Neuroscience·Kai KailaJuha Voipio
Jun 10, 2015·The Journal of Cell Biology·Olaya LlanoAnastasia Ludwig
Oct 7, 2015·Frontiers in Cellular Neuroscience·Vivek MahadevanMelanie A Woodin
Dec 4, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Quentin ChevyJean Christophe Poncer
Jan 7, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xin TangGong Chen
Jul 7, 2017·Cell Reports·Gina Rinetti-VargasKevin J Bender
Dec 5, 2017·Progress in Neurobiology·Graziella Di CristoMassimo Avoli

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Citations

Feb 14, 2019·The Journal of Physiological Sciences : JPS·Kayo NakamuraJunichi Nabekura
Jun 9, 2020·Frontiers in Molecular Neuroscience·Russell J TaylorErik W Dent
Jun 17, 2020·Cell and Tissue Research·Marta ZagrebelskyMartin Korte
Aug 10, 2019·Frontiers in Molecular Neuroscience·Yvonne E MooreStephen J Moss
Feb 8, 2021·Neuroscience and Biobehavioral Reviews·Carlijn Peerboom, Corette J Wierenga
Feb 5, 2021·The Journal of Biological Chemistry·Georgina KontouJoshua L Smalley
Mar 1, 2021·Trends in Neurosciences·Mari A VirtanenKai Kaila
Mar 16, 2019·Neuropharmacology·Etienne CômeSabine Lévi

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