KCNMA1 Expression is Downregulated in Colorectal Cancer via Epigenetic Mechanisms

Cancers
Maria Sofia BasileAntonio Pesce

Abstract

KCNMA1 is a gene located at 10q22 that encodes the pore-forming α-subunit of the large-conductance Ca2+-activated K⁺ channel. KCNMA1 is down-regulated in gastric carcinoma tumors, through hypermethylation of its promoter. In the present study, we have evaluated the expression levels of KCNMA1 both in a mouse model of Colorectal Cancer (CRC) and in human CRC samples. Additionally, epigenetic mechanisms of KCNMA1 gene regulation were investigated. We observed a significant down-regulation of KCNMA1 both in a human and mouse model of CRC. No differences in KCNMA1 levels were, however, observed at different TNM stages. We also wanted to determine whether the modulation in KCNMA1 was dependent on epigenetic mechanisms. A statistically significant inverse correlation between KCNMA1 expression and mir-17-5p levels was observed in patients with CRC. Furthermore, in the tumor samples, we found a significant hypermethylation of the promoter, in the loci cg24113782 and cg25655799, compared to healthy tissue. Overall, our data suggest the possible use of KCNMA1 as a therapeutic target in the early stages of CRC.

References

Jul 31, 2009·BMC Cancer·Divya KhaitanNagendra S Ningaraj
Apr 28, 2010·Gastroenterology·C Richard Boland, Ajay Goel
Apr 13, 2011·Journal of Carcinogenesis·Mariangela De RobertisVito Michele Fazio
May 6, 2011·International Journal of Cancer. Journal International Du Cancer·Pia AlhopuroLauri A Aaltonen
Aug 18, 2012·PloS One·Martin OeggerliLukas Bubendorf
Jul 17, 2014·International Immunopharmacology·Lisheng GeMartin R Jadus
Sep 21, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Priya SamuelDavid Raul Francisco Carter

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Citations

Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eugenio CavalliMaria Cristina Petralia
Sep 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonio PesceKatia Mangano
Jan 4, 2021·Breast Cancer Research and Treatment·Zhifa ZhangXiaofeng Dai

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Datasets Mentioned

BETA
GSE24514
and
GSE32323
GSE35834
GSE53051
GSE57341
GSE57342

Methods Mentioned

BETA
Methylation
transfection
PCA
sumoylation
xenograft
RNA-seq
biopsies

Software Mentioned

RSEM
MAS5

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