KCNQ1OT1/miR-217/ZEB1 feedback loop facilitates cell migration and epithelial-mesenchymal transition in colorectal cancer

Cancer Biology & Therapy
Yinzhu BianXiaoping Qian

Abstract

Long noncoding RNAs are widely acknowledged as a group of regulatory factors in various diseases, especially in cancers. KCNQ1 overlapping transcript 1 (KCNQ1OT1) has been reported as oncogene in human cancers. However, the role of KCNQ1OT1 in colorectal cancer (CRC) has not been fully explained. Based on the database analysis, KCNQ1OT1 was highly expressed in CRC samples and predicted the poor prognosis for CRC patients. Functional experiments revealed that KCNQ1OT1 knockdown negatively affected the proliferation, migration and epithelial-mesenchymal transition (EMT) in CRC cells. Moreover, we identified the cytoplasmic localization of KCNQ1OT1 in CRC cells, indicating the post-transcriptional regulation of KCNQ1OT1 on gene expression. Mechanism experiments including RNA Immunoprecipitation (RIP) assay and dual luciferase reporter assays verified that KCNQ1OT1 acted as a competing endogenous RNA (ceRNA) in CRC by sponging microRNA-217 (miR-217) to up-regulate the expression of zinc finger E-box binding homeobox 1 (ZEB1). Further mechanism investigation revealed that ZEB1 enhanced the transcription activity of KCNQ1OT1 by acting as a transcription activator. Finally, rescue assays were designed to demonstrate the effect of KCNQ...Continue Reading

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Citations

May 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sen LinHongbo Wang
May 8, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yanyan WuYajie Zhang
Aug 30, 2019·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Yu LiangDong-Qiu Dai
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Jan 23, 2020·Journal of Cellular and Molecular Medicine·Qun ZhangXiaoping Qian
Jul 31, 2020·BMC Medical Genomics·Arash PoursheikhaniMohammad Amin Kerachian
Jan 17, 2021·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Yilei DengLongshuan Zhao
Dec 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shuai ZhouHui Chun Liu
Dec 2, 2021·Journal of Cellular and Molecular Medicine·Yushan LiMing Liu

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Methods Mentioned

BETA
transfection
protein assay
electrophoresis
Immunoprecipitation
RIP
pull-down
PCR
transfecting
Pull down
ChIP

Software Mentioned

JASPAR
miRandaSites
PITASites
Quantity One

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