KCNQ2/3/5 channels in dorsal root ganglion neurons can be therapeutic targets of neuropathic pain in diabetic rats

Molecular Pain
Ting YuZhenzhong Li

Abstract

Background Diabetic neuropathic pain is poorly controlled by analgesics, and the precise molecular mechanisms underlying hyperalgesia remain unclear. The KCNQ2/3/5 channels expressed in dorsal root ganglion neurons are important in pain transmission. The expression and activity of KCNQ2/3/5 channels in dorsal root ganglion neurons in rats with diabetic neuropathic pain were investigated in this study. Methods The mRNA levels of KCNQ2/3/5 channels were analyzed by real-time polymerase chain reaction. The protein levels of KCNQ2/3/5 channels were evaluated by Western blot assay. KCNQ2/3/5 channel expression in situ in dorsal root ganglion neurons was detected by double fluorescent labeling technique. M current (IM) density and neuronal excitability were determined by whole-cell voltage and current clamp recordings. Mechanical allodynia and thermal hyperalgesia were assessed by von Frey filaments and plantar analgesia tester, respectively. Results The mRNA and protein levels of KCNQ2/3/5 channels significantly decreased, followed by the reduction of IM density and elevation of neuronal excitability of dorsal root ganglion neurons from diabetic rats. Activation of KCNQ channels with retigabine reduced the hyperexcitability and inhi...Continue Reading

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Citations

May 30, 2019·International Journal of Molecular Sciences·Isabella SalzerStefan Boehm
Feb 26, 2019·Future Medicinal Chemistry·Christian Bock, Andreas Link
Dec 18, 2019·Brain Research·Nathalia Maria Silva-Dos-SantosJosé Henrique Leal-Cardoso
May 29, 2021·RSC Medicinal Chemistry·Matteo BorginiPeter Wipf
Jun 23, 2021·The Journal of Pain : Official Journal of the American Pain Society·Georgina Palomés-BorrajoClara Penas
Jun 22, 2021·Frontiers in Molecular Neuroscience·Deepanjali Dwivedi, Upinder S Bhalla
Aug 28, 2021·Life·Shenglan Wang, Yi Dai

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Methods Mentioned

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