KDM2B overexpression correlates with poor prognosis and regulates glioma cell growth

OncoTargets and Therapy
Yiwei WangXiaojie Wang

Abstract

Gliomas are one of the most lethal cancers in the human central nervous system. Despite clinical treatment advancements, the prognosis of patients with glioma remains poor. KDM2B is a histone lysine demethylase, which has been observed in multiple tumors. But the concrete role of KDM2B in gliomas remains to be further illustrated. The KDM2B expression in gliomas was detected with immunohistochemistry and Western blot assay. Furthermore, knockdown of KDM2B in U87 and U251 glioma cell lines, the proliferation capacity was evaluated by cell viability assay, colon formation assay and flow cytometry in vitro. Western blot assay was used to analyze the p21, EZH2 and cyclinD1 changes followed by knockdown of KDM2B. KDM2B was upregulated in tissues of glioma patients, and the expression was correlated to cancer progression. Downregulation of KDM2B in U87 and U251 glioma cell lines inhibited cell proliferation and arrested cell cycle in G0/G1 phase. In addition, silencing KDM2B promoted the upregulation of p21 while reduced the expression of EZH2 and cyclinD1. Taken together, our results revealed that KDM2B might influence gliomas growth and act as a novel therapeutic target for glioma patients.

Citations

Sep 19, 2019·Journal of Cellular Physiology·Yuxi Qi, Yanning Zhao
Dec 20, 2020·Ageing Research Reviews·Maria M Bayliak, Volodymyr I Lushchak
Nov 5, 2020·Pharmacology & Therapeutics·Elena KunadisChristina Piperi
Apr 2, 2021·Frontiers in Oncology·Jaceline Gisliane Pires SanchesLianhong Li
Apr 14, 2021·Nature Chemical Biology·Ryan J WeissJeffrey D Esko
Jul 6, 2021·Frontiers in Genetics·Gaya Punnia-MoorthyJessamy Tiffen
Aug 8, 2021·International Journal of Molecular Sciences·Qiong WuArnold B Etame

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Methods Mentioned

BETA
electrophoresis
Assay
flow cytometry
transfection

Software Mentioned

ImageJ
SPSS

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