Keloid-derived keratinocytes acquire a fibroblast-like appearance and an enhanced invasive capacity in a hypoxic microenvironment in vitro

International Journal of Molecular Medicine
Xiao-Yang MaXiaojun Wang

Abstract

A keloid scar is an overgrowth of dense fibrous tissue that develops around a wound. These scars are raised scars that spread beyong the margins of the orinigal wound to normal skin by invasion. Keloid tissue consists of both an epithelium and dermal fibroblasts. Recent studies have primarily focused on keloid fibroblasts; however, the precise role of keratinocytes in the invasion process of keloids remains to be identified. Hypoxia is a typical characteristic of keloid scars, as well as other solid tumors. The expression of the transcription factor, hypoxia-inducible factor-1α (HIF-1α), is mainly induced by hypoxia and is known for its ability to induce proliferative and transformative changes in cells; its expression has been shown to correlate with tumor invasion and metastasis. In the present study, we used immunohistochemistry, fluorescence staining and western blot analysis and demonstrated that HIF-1α was highly expressed in both the epithelial layer of keloid tissue specimens and in hypoxia-exposed keratinocytes, which suggested that the keloid keratinocytes underwent epithelial-to-mesenchymal transition (EMT) in vitro. The high expression of mesenchymal markers, such as as vimentin and fibronectin was confirmed, as wel...Continue Reading

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Citations

Feb 15, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Maribanyana LebekoArdeshir Bayat
Jul 3, 2019·The Journal of Clinical Investigation·Joanne C MastersonGlenn T Furuta
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Jun 5, 2020·Journal of Plastic Surgery and Hand Surgery·Loubin SiXiaojun Wang
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Jun 21, 2019·Cells·Alexandra L RippaEkaterina A Vorotelyak
Jan 11, 2016·Lasers in Surgery and Medicine·Qingliang LiuBo Chen
Dec 18, 2020·Frontiers in Immunology·Jianni WuEmma Guttman-Yassky
Jan 26, 2021·Biomaterials·Kelsey G DeFratesPhillip B Messersmith
Mar 7, 2019··Francesco PavoneDuccio Rossi Degl'Innocenti

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Methods Mentioned

BETA
biopsies
transfection
biopsy
phase contrast microscopy

Software Mentioned

SPSS Statistics

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