Keratin 17 is co-expressed with 14-3-3 sigma in oral carcinoma in situ and squamous cell carcinoma and modulates cell proliferation and size but not cell migration

Virchows Archiv : an International Journal of Pathology
Toshihiko MikamiTakashi Saku

Abstract

Expression of keratin (K) 13 is replaced with that of K17 when squamous cells of the oral mucosa transform from normal and dysplastic epithelia to carcinoma in situ (CIS) and squamous cell carcinoma (SCC). Since 14-3-3 sigma is functionally associated with K17, we examined possible relationships between expression of K17 and 14-3-3 sigma in oral CIS and SCC tissues by immunohistochemistry. We furthermore examined whether or not K17 expression or knockdown by small interfering RNA (siRNA) modulates the behavior of SCC cells in culture in terms of cell proliferation and migration. In tissue specimens of oral SCC and CIS, the pattern of cytoplasmic expression of 14-3-3 sigma and K17 was similar but neither was expressed in normal or dysplastic epithelia. Both proteins were demonstrated in the cytoplasm of control oral SCC ZK-1 cells, but expression of 14-3-3 sigma changed from cytoplasmic to nuclear upon knockdown of K17. In carcinoma cells, therefore, cytoplasmic localization of 14-3-3 sigma seems to accompany expression of K17. In K17-knockdown cells, proliferation was significantly suppressed at 4 days after seeding. In addition, the cell size of K17-knockdown cells was significantly smaller than that of control cells; as a res...Continue Reading

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Citations

Aug 12, 2016·PloS One·Rumana KhanomKei Sakamoto
Nov 18, 2017·Veterinary Pathology·Alejandro Suárez-BonnetSimon L Priestnall
Oct 12, 2018·The Journal of Pathology·Luting YangGang Wang
Mar 28, 2017·Journal of Cancer Research and Clinical Oncology·Yurie MikamiTamotsu Kiyoshima
Jan 1, 2020·Clinical and Experimental Dental Research·Manabu ShigeokaHiroshi Yokozaki
Jan 29, 2020·Oncogene·Olivia McGinnCarol A Sartorius
Apr 16, 2021·The International Journal of Lower Extremity Wounds·Joon M JungChong H Won
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Alexandros HardasSimon L Priestnall
Jul 8, 2021·Seminars in Cell & Developmental Biology·Yiting LinGang Wang
Aug 14, 2021·RNA Biology·Erika Pereira ZambaldeJaqueline Carvalhode Oliveira

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