PMID: 6169731Aug 1, 1981Paper

Keratin filaments of mouse epithelial cells are rapidly affected by epidermal growth factor

The Journal of Cell Biology
J Keski-OjaI Virtanen

Abstract

The effects of epidermal growth factor (EGF) on the cytokeratin filaments of cultured murine epithelial cells were studied by the indirect immunofluorescence technique with affinity-purified antibodies. Mouse epithelial cells (MMC-E), grown on glass cover slips, and viewed by immunofluorescence microscopy, showed keratin-specific fluorescence as typical bright perinuclear aggregates corresponding to dense paracrystalline granules seen in electron microscopy. Within minutes after an exposure to EGF, the keratin granules in the MMC-E cells decreased. After 10 min of incubation, the cells had spread fibrillar keratin. Such an effect could not be found after a similar exposure to insulin, dexamethasone, dibutyryl cyclic AMP, or antimitotic drugs. EGF, therefore, has a relatively direct effect on the cytoskeletal organization of cultured epithelial cells. These rapid effects on the keratin filaments may explain the simultaneous EGF-induced ultrastructural surface changes of the cells. EGF may thus function as a regulatory factor in the migration of epithelial cells and in the mobility of their cell membranes. The epithelial cell line, MMC-E, should prove a useful model for studies on the action of EGF on nontransformed epithelial ce...Continue Reading

References

Oct 1, 1979·The Journal of Cell Biology·H T HaiglerS Cohen
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1976·Journal of Cellular Physiology·G Carpenter, S Cohen
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Jul 1, 1980·Journal of Cellular Physiology·J Keski-OjaG J Todaro

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Citations

Nov 1, 1983·Developmental Biology·E LehtonenI Virtanen
Jun 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Marcin MochReinhard Windoffer
Jan 12, 2012·The Journal of Biological Chemistry·Byung-Min ChungPierre A Coulombe
Aug 1, 1990·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·A NakahataY Hayashi
Oct 1, 1988·European Journal of Pediatrics·M Koskiniemi
Dec 1, 1981·Journal of Ultrastructure Research·U I HeineB Wetzel
Jun 1, 1983·Journal of Cellular Physiology·K AlitaloP Bornstein
Oct 1, 1981·Prenatal Diagnosis·H von KoskullP Aula
Jun 1, 1992·Differentiation; Research in Biological Diversity·K FröjdmanL J Pelliniemi
Jul 28, 2013·Current Opinion in Cell Biology·Byung-Min ChungPierre A Coulombe
Aug 1, 1982·Journal of Clinical Microbiology·J Keski-Oja, J Paavonen
Jan 1, 1984·The Journal of Investigative Dermatology·P FleckmanJ McGuire

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