Keratin protein expression during the development of conducting airway epithelium in nonhuman primates.

Differentiation; Research in Biological Diversity
T H HuangR Wu

Abstract

Keratin protein expression during the development of Rhesus monkey conducting airway epithelium was investigated by both biochemical and immunohistochemical methods. Keratin proteins were extracted from tracheal and intrapulmonary airway tissues of fetal (at 80- and 140-day gestational ages), neonatal, and adult animals. Using immunoblot analyses and immunohistochemistry with various monoclonal (AE1, AE3, AE8, 6.01 and 6.11) and monospecific antibodies (anti-50/55 and anti-40 kDa), the presence of keratins 5, 6, 8, 13, 14, and 19 in adult airway epithelium were demonstrated. Except for keratin 13 (51 kDa), the remaining keratins could be immunologically detected in fetal and neonatal tissues. To further understand the nature of the synthesis of keratin 13 during development, airway epithelial cells from different ages were isolated and cultured in vitro. Cultured cells were labeled with 35S-methionine, and the patterns of keratin protein were analyzed by one- and two-dimensional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Results indicated that the cultured airway cells synthesized additional keratins including 7, 15, 16, 17, and 18. However, consistent with the in vivo finding, fetal cells synthesized less...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1977·The American Review of Respiratory Disease·R G Breeze, E B Wheeldon
Oct 1, 1985·The Journal of Cell Biology·B A DaleT T Sun
Jan 1, 1985·Annals of the New York Academy of Sciences·T T SunR Eichner
Jan 1, 1987·Differentiation; Research in Biological Diversity·M RodriguesT T Sun
Jul 1, 1988·The American Journal of Anatomy·N K TylerC G Plopper
Nov 1, 1982·The Journal of Cell Biology·J Woodcock-MitchellT T Sun
Jul 1, 1983·The Journal of Investigative Dermatology·T T SunJ Woodcock-Mitchell
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·W W FrankeK Illmensee

❮ Previous
Next ❯

Citations

Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·M J Malecha, M Miettinen
Feb 20, 1998·European Journal of Oral Sciences·M Feghali-AssalyJ P Ouhayoun
May 1, 1993·American Journal of Respiratory Cell and Molecular Biology·S H RandellP Nettesheim
Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·R E Leube, T J Rustad
Aug 19, 2006·American Journal of Respiratory and Critical Care Medicine·Steven R Hays, John V Fahy
Jan 19, 2008·Avian Pathology : Journal of the W.V.P.A·K M ZaffutoC L Afonso
Aug 1, 1992·The Journal of Investigative Dermatology·M OhtsukiM Blumenberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.