Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis

Journal of Zhejiang University. Science. B
Cheng-gang CaiXiao-dong Zheng

Abstract

A new feather-degrading bacterium was isolated from a local feather waste site and identified as Bacillus subtilis based on morphological, physiochemical, and phylogenetic characteristics. Screening for mutants with elevated keratinolytic activity using N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis resulted in a mutant strain KD-N2 producing keratinolytic activity about 2.5 times that of the wild-type strain. The mutant strain produced inducible keratinase in different substrates of feathers, hair, wool and silk under submerged cultivation. Scanning electron microscopy studies showed the degradation of feathers, hair and silk by the keratinase. The optimal conditions for keratinase production include initial pH of 7.5, inoculum size of 2% (v/v), age of inoculum of 16 h, and cultivation at 23 degrees C. The maximum keratinolytic activity of KD-N2 was achieved after 30 h. Essential amino acids like threonine, valine, methionine as well as ammonia were produced when feathers were used as substrates. Strain KD-N2, therefore, shows great promise of finding potential applications in keratin hydrolysis and keratinase production.

References

Aug 1, 1987·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·K WawrzkiewiczT Wolski
Dec 11, 1999·Extremophiles : Life Under Extreme Conditions·H TakamiK Horikoshi
Mar 10, 2000·Applied Microbiology and Biotechnology·H GradisarJ Friedrich
Apr 5, 2003·Archives of Microbiology·Alessandro RiffelAdriano Brandelli
Apr 12, 2003·Bioresource Technology·W Suntornsuk, L Suntornsuk
Nov 26, 2003·The Journal of Infectious Diseases·Jan P M LangeveldJason C H Shih
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Jan 11, 2005·Applied and Environmental Microbiology·Alexandre J MacedoCarlos Termignoni
Jun 1, 1990·Applied and Environmental Microbiology·C M WilliamsJ C Shih
Jan 5, 2006·Applied Microbiology and Biotechnology·Rani Gupta, Priya Ramnani

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Citations

Dec 30, 2009·Applied Microbiology and Biotechnology·Adriano BrandelliAlessandro Riffel
Oct 15, 2013·Applied Microbiology and Biotechnology·Rani GuptaNamita Gupta
Apr 8, 2009·Journal of Industrial Microbiology & Biotechnology·Chenggang Cai, Xiaodong Zheng
Oct 15, 2009·Applied Biochemistry and Biotechnology·Mervat Morsy A El-Gendy
Feb 19, 2013·BMC Biotechnology·Ana Lúcia Vazquez VillaAlane Beatriz Vermelho
Aug 14, 2013·Critical Reviews in Biotechnology·Daniel Joner Daroit, Adriano Brandelli
May 31, 2012·Critical Reviews in Biotechnology·Rani GuptaQasim K Beg
Sep 25, 2012·Journal of Bioscience and Bioengineering·Wei-Hsun LoJane-Yii Wu
Jul 17, 2015·BioMed Research International·Subash C B GopinathM K Md Arshad
Sep 3, 2008·Journal of Zhejiang University. Science. B·Cheng-gang CaiXiao-dong Zheng
Jan 7, 2015·British Poultry Science·N Sivakumar, S Raveendran
Sep 19, 2012·Waste Management·Adeseye LasekanDzulkifly Hashim
Jan 18, 2015·Waste Management·Yun XiaDaniel I Massé
Jan 8, 2016·Biotechnology, Biotechnological Equipment·Agbaje LateefEvariste Bosco Gueguim-Kana
Jun 14, 2016·Critical Reviews in Biotechnology·Amit VermaGurpreet Singh Dhilon
Jun 29, 2016·Applied Biochemistry and Biotechnology·Regina J PatinvohMohammad J Taherzadeh
Mar 15, 2011·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T R DuarteA B Vermelho
Oct 26, 2018·Journal of Basic Microbiology·K TamreihaoDebananda S Ningthoujam
Feb 1, 2020·PloS One·Poonam NasipuriSøren J Sørensen
Dec 24, 2019·Frontiers in Microbiology·Qingxin Li
Feb 9, 2019·Journal, Genetic Engineering & Biotechnology·Azza M Abdel-FattahAmal M Hashem
Sep 19, 2017·Applied Biochemistry and Biotechnology·Priya FukeAnshuman A Khardenavis
Dec 18, 2018·3 Biotech·Daniel Pereira de PaivaSelma Soares de Oliveira
Feb 8, 2021·The Protein Journal·Ozlem Karaveli, Irem Deniz
Jan 5, 2021·Frontiers in Microbiology·Nonso E NnolimUchechukwu U Nwodo
Jun 7, 2021·Protein Expression and Purification·Elebert Pauline MwanzaCelia Hugo
Jul 13, 2021·Frontiers in Microbiology·Qingxin Li
Jul 29, 2021·Brazilian Journal of Biology = Revista Brasleira De Biologia·S MasoodS Sattar

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