Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields

Bioelectrochemistry
Xi RenJiaping Zhang

Abstract

Endogenous electric fields (EFs) direct the migration (electrotaxis) of keratinocytes in skin wounds, and the exogenous application of EFs may therefore improve wound healing, but the potential benefits are limited by the side effects of constant direct current (DC) passing through tissues. In contrast, with pulsed DC (characterized by intermittent output), parameters can be adjusted to minimize the adverse effects of electric currents. However, it remains unknown whether pulsed DC can reliably induce keratinocyte electrotaxis. In this study, using primary keratinocytes in an electrotaxis chamber, we found that a pulsed DCEF at physiological strength (EF = 150 mV/mm, duty cycle = 60%, frequency = 0.1 Hz) could induce robust electrotaxis. This effect was dependent on both voltage and duty cycle, but not on frequency. As predicted, fewer electrochemical reactions and cytotoxic reactions were detected with pulsed DCEF than with constant DCEF. In summary, we here demonstrate for the first time, that pulsed DCEF can trigger keratinocyte electrotaxis comparable to that induced by constant DCEF, while minimizing the electrochemical side effects. These findings support the future development of a pulsed DCEF device to improve wound hea...Continue Reading

Citations

Sep 2, 2020·Advances in Wound Care·Paulo Luiz FarberLydia Masako Ferreira
Apr 16, 2020·Bioelectricity·Samantha L PayneMadeleine J Oudin
Jan 25, 2020·Cellular and Molecular Life Sciences : CMLS·Siwei ZhaoMin Zhao
Jul 16, 2021·Biosensors & Bioelectronics·Tom J ZajdelDaniel J Cohen
Aug 24, 2021·Frontiers in Immunology·Maryam MoarefianCaroline N Jones
Feb 4, 2022·International Wound Journal·Gianluca MelottoJacqueline Rachel Forss

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