Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin 5

American Journal of Respiratory Cell and Molecular Biology
Z BorokE D Crandall

Abstract

We investigated the role of keratinocyte growth factor (KGF) in regulation of alveolar epithelial cell (AEC) phenotype in vitro. Effects of KGF on cell morphology, expression of surfactant apoproteins A, B, and C (SP-A, -B, and -C), and expression of aquaporin 5 (AQP5), a water channel present in situ on the apical surface of alveolar type I (AT1) cells but not expressed in alveolar type II (AT2) cells, were evaluated in AECs grown in primary culture. Observations were made on AEC monolayers grown in serum-free medium without KGF (control) or grown continuously in the presence of KGF (10 ng/ml) from either Day 0 (i.e., the time of plating) or Day 4 or 6 through Day 8 in culture. AECs monolayers express AQP5 only on their apical surfaces as determined by cell surface biotinylation studies. Control AECs grown in the absence of KGF through Day 8 express increasing levels of AQP5, consistent with transition toward the AT1 cell phenotype. Exposure of AECs to KGF from Day 0 results in decreased AQP5 expression, retention of a cuboidal morphology, and greater numbers of lamellar bodies relative to control on Day 8 in culture. AECs treated with KGF from Day 4 or 6 exhibit a decrease in AQP5 expression through subsequent days in culture...Continue Reading

References

Feb 1, 1975·Experimental and Molecular Pathology·M J EvansG Freeman
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1992·The American Journal of Physiology·F StahlM Wiederholt
Mar 1, 1992·American Journal of Respiratory Cell and Molecular Biology·S I DantoE D Crandall
Jan 15, 1991·The Biochemical Journal·T E Weaver, J A Whitsett
Oct 1, 1989·Experimental Cell Research·J M CheekE D Crandall
Oct 14, 1982·Biochimica Et Biophysica Acta·L G DobbsK Sueishi
Mar 1, 1994·The Journal of Experimental Medicine·G F PierceC F Morris
Oct 1, 1995·The Journal of Clinical Investigation·R J PanosL J Smith
May 1, 1995·Cell Biology International·J S RubinP W Finch
May 1, 1995·American Journal of Respiratory Cell and Molecular Biology·S I DantoE D Crandall
Jan 1, 1995·American Journal of Respiratory Cell and Molecular Biology·Z BorokE D Crandall
Jan 1, 1993·American Journal of Respiratory Cell and Molecular Biology·P J ChristensenR Paine
Mar 1, 1994·The Journal of Clinical Investigation·T R UlichG F Pierce
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·W S SimonetJ A Whitsett
Sep 1, 1996·American Journal of Respiratory Cell and Molecular Biology·M ShiratoriS L Katyal
Oct 1, 1996·American Journal of Respiratory Cell and Molecular Biology·T YanoR J Mason
Jan 1, 1997·Pflügers Archiv : European journal of physiology·X HeB J Baum

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Citations

Nov 18, 2010·In Vitro Cellular & Developmental Biology. Animal·Hong-Yan LuHong Wang
Sep 10, 2002·Trends in Endocrinology and Metabolism : TEM·Landon S King, Masato Yasui
May 23, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·L G Dobbs, J A Gutierrez
Oct 26, 1999·The International Journal of Biochemistry & Cell Biology·L J ReynoldsR J Richards
Feb 20, 2007·Molecular BioSystems·Meshell D Johnson
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M KraneA G Menon
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·L S King
Sep 5, 2008·American Journal of Physiology. Cell Physiology·Beiyun ZhouZea Borok
May 2, 2008·American Journal of Physiology. Cell Physiology·Beiyun ZhouZea Borok
Mar 31, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lucas DemaioZea Borok
Sep 14, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael J LaFeminaJames A Frank
Oct 20, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zea Borok, Edward D Crandall
Apr 7, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lynn M Crosby, Christopher M Waters
Apr 13, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Philip L BallardMichael A Matthay
Jun 23, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Chuanxiu YangLin Liu
Sep 1, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert F GonzalezLeland G Dobbs
Apr 3, 2001·American Journal of Respiratory and Critical Care Medicine·E D Crandall, M A Matthay
Feb 8, 2003·American Journal of Respiratory and Critical Care Medicine·Antonia FehrenbachHeinz Fehrenbach
Dec 17, 2009·American Journal of Respiratory and Critical Care Medicine·Ali O YildirimHeinz Fehrenbach
Dec 30, 1999·American Journal of Respiratory Cell and Molecular Biology·L S KingP Agre
Mar 1, 2000·American Journal of Respiratory Cell and Molecular Biology·M I RamirezM C Williams
Feb 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Fushan WangMichael Koval
Feb 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Renli QiaoZea Borok
May 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Brant E IsaksonScott Boitano
Jul 23, 2004·American Journal of Respiratory Cell and Molecular Biology
Aug 2, 2005·American Journal of Respiratory Cell and Molecular Biology·Kahoru NishinaRobert J Mason
Mar 4, 2006·American Journal of Respiratory Cell and Molecular Biology·Hans G Folkesson, Michael A Matthay
Jun 24, 2006·American Journal of Respiratory Cell and Molecular Biology·Kevin A LeinerPhilip L Sannes
Mar 3, 2007·American Journal of Respiratory Cell and Molecular Biology·Brigitte N GompertsRobert M Strieter
Sep 22, 2007·American Journal of Respiratory Cell and Molecular Biology·Sandra R BatesGeorge H Rothblat
Sep 18, 2007·American Journal of Respiratory Cell and Molecular Biology·Renli QiaoZea Borok
May 9, 2009·American Journal of Respiratory Cell and Molecular Biology·Michael KovalJesse Roman
Sep 22, 2009·American Journal of Respiratory Cell and Molecular Biology·Per FlodbyEdward D Crandall
Jul 10, 2010·American Journal of Respiratory Cell and Molecular Biology·Shaohua Wang, Rolf D Hubmayr
Jan 5, 2000·The Journal of Clinical Investigation·T MaA S Verkman
Jan 5, 2000·The Journal of Clinical Investigation·L S KingP Agre
Jul 30, 2009·Journal of Leukocyte Biology·Tanya A Miura, Kathryn V Holmes
Mar 22, 2006·Pediatric Research·Zea BorokParviz Minoo

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