PMID: 7528248Jan 1, 1995Paper

Keratinocyte muscarinic acetylcholine receptors: immunolocalization and partial characterization

The Journal of Investigative Dermatology
S A GrandoM V Dahl

Abstract

We have reported previously that human keratinocytes synthesize and secrete acetylcholine and that muscarinic cholinergic drugs have effects on keratinocyte proliferation, adhesion, and migration. This study defines the location of muscarinic acetylcholine receptors in human epidermis and describes some pharmacologic and molecular properties of these receptors. Confocal microscopy employing the anti-muscarinic receptor monoclonal antibody M35 visualized the receptors in the intercellular areas of normal human epidermis. Using immunoelectron microscopy, the receptors appeared to be attached to the keratinocyte plasma membranes. Functional, high-density (Bmax = 8.3 nmol/2 x 10(6) cells) and high-affinity (Kd = 21.5 nM) muscarinic receptors were demonstrated by saturable binding of the reversible radioligand [3H]quinuclidinyl benzilate to the surfaces of freshly isolated epidermal cells at 0 degrees C. Receptor proteins were separated by gel electrophoresis. An apparent isoelectric point of pH 4.3 was determined in immunoblots of sodium-cholate-solubilized receptors separated on isoelectric-focusing gels. Three protein bands, two at approximately 60 kDa and one at 95 kDa, were visualized in immunoblots of membrane-bound or solubil...Continue Reading

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Citations

Mar 22, 2001·Experimental Dermatology·D GroeneG Heyer
Mar 7, 2001·The Journal of Clinical Endocrinology and Metabolism·S FritzA Mayerhofer
Mar 13, 2001·Japanese Journal of Pharmacology·I WesslerC J Kirkpatrick
Oct 27, 2004·Experimental Dermatology·Laurent Misery
Oct 4, 2006·Physiological Reviews·Dirk RoostermanMartin Steinhoff
Apr 16, 2002·Experimental Dermatology·Sonja Ständer, Martin Steinhoff
Oct 28, 2004·Experimental Dermatology·Hjalmar Kurzen, Karin U Schallreuter
Jul 5, 2006·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Koichiro Kawashima
Mar 19, 1999·Clinical and Experimental Pharmacology & Physiology·I WesslerK Racké
Apr 14, 2010·Allergy·J Buddenkotte, M Steinhoff
Dec 31, 1997·The Journal of Dermatology·G HeyerO P Hornstein
Nov 15, 2006·Autoimmunity·Hjalmar Kurzen, Sarah Brenner
Jan 29, 2005·American Journal of Clinical Dermatology·Rachel Grossman
Apr 20, 2000·European Journal of Pharmacology·B M Conti-FineA D Maus
Mar 29, 2002·The Journal of Investigative Dermatology·T MauroE Hummler
Jan 10, 2002·Pulmonary Pharmacology & Therapeutics·I K Wessler, C J Kirkpatrick
Oct 31, 2002·The Journal of Investigative Dermatology·Rainer Viktor HaberbergerWolfgang Kummer
Feb 12, 2004·The Journal of Investigative Dermatology. Symposium Proceedings·Sergei A Grando
Mar 17, 2004·Experimental Cell Research·Vu Thuong NguyenSergei A Grando
Dec 1, 1995·The Journal of Investigative Dermatology·S A GrandoB M Conti-Fine
Oct 16, 2004·The Journal of Investigative Dermatology·Hjalmar KurzenMartin Deichmann
Jun 3, 2006·The Journal of Investigative Dermatology·Benjamin BeckRoman Skryma
Jul 1, 2006·The Journal of Investigative Dermatology·Hjalmar KurzenNicole Maas-Szabowski

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