Keratinocyte proinflammatory responses to adherent and nonadherent group A streptococci.

Infection and Immunity
B WangM Caparon

Abstract

The gram-positive bacterium Streptococcus pyogenes (group A streptococcus) is the causative agent of a wide variety of suppurative infections of cutaneous tissues. Previous analyses have demonstrated that the M protein of S. pyogenes is an adhesin that directs the attachment of the streptococcus to keratinocytes in the skin. In this study, we have examined keratinocyte function in response to S. pyogenes and found that adherent versus nonadherent streptococci promote distinct patterns of expression of several proinflammatory molecules and keratinocyte cell fate. When analyzed by a quantitative reverse transcriptase PCR method, infection of cultured HaCaT keratinocytes with adherent, but not nonadherent, streptococci resulted in increased expression of mRNA for the cytokines interleukin-1alpha (IL-1alpha), IL-1beta, and IL-8 but neither infection induced expression of tumor necrosis factor alpha. In contrast, both adherent and nonadherent S. pyogenes induced expression of IL-6 and each promoted synthesis and release of prostaglandin E2 (PGE2). However, considerably greater levels of IL-6 expression were stimulated by adherent streptococci relative to nonadherent streptococci and the kinetics of PGE2 release in response to nonadh...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
May 1, 1991·Infection and Immunity·M G CaparonJ R Scott
Jun 1, 1990·The Journal of Investigative Dermatology·J AnselS Hefeneider
Jun 1, 1990·The Journal of Investigative Dermatology·P B Sehgal
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R M GrossmanA B Gottlieb
Jul 1, 1989·Clinical Microbiology Reviews·V A Fischetti
Nov 1, 1986·The Journal of Experimental Medicine·J R ScottV A Fischetti
Jan 1, 1970·The New England Journal of Medicine·L W Wannamaker
Jun 1, 1967·Journal of Bacteriology·R W Quinn, P N Lowry
Jul 1, 1995·Trends in Microbiology·S R HedgesC Svanborg
Nov 1, 1995·The Journal of General Virology·S KranticC Rabourdin-Combe
Mar 1, 1995·Molecular Microbiology·J Perez-CasalJ R Scott
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·V KapurJ M Musser
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N OkadaM Caparon
Aug 1, 1993·Infection and Immunity·L N HeissW E Goldman
Jan 25, 1996·The New England Journal of Medicine·A L Bisno, D L Stevens
Mar 1, 1964·Archives of Dermatology·H J WHYTE, R D BAUGHMAN

❮ Previous
Next ❯

Citations

Apr 18, 2000·Infection and Immunity·D A ScaramuzzinoD E Bessen
Jul 3, 2002·Clinical and Diagnostic Laboratory Immunology·Yuka NakamuraShinichi Watanabe
Jun 10, 2004·Molecular Microbiology·Michael A Meehl, Michael G Caparon
Sep 24, 2005·Infection and Immunity·Angela L BrickerMichael R Wessels
Feb 26, 2013·PloS One·Katariina Hannula-JouppiJuha Kere
Oct 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jamie J Bernard, Richard L Gallo
Feb 23, 2000·FEMS Immunology and Medical Microbiology·I NuzzoC R Carratelli
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
Feb 16, 2019·Microbiology Spectrum·Vijay Pancholi
Jul 25, 2000·Clinical Microbiology Reviews·M W Cunningham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.