PMID: 8601723Feb 1, 1996Paper

Keratinocytes-associated chemokines and enzymatically quiescent heparanase induce the binding of resting CD4+ T cells

The Journal of Investigative Dermatology
R HershkovizO Lider

Abstract

Whether the chemokines macrophage inflammatory protein-1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted (RANTES), which interact specifically with glycosaminoglycans and thus mediate the recruitment, attachment, and migration of leukocytes to vascular endothelia and extracellular matrix, are also involved in interactions between CD4+ murine T lymphocytes and keratinocytes was examined. We have previously observed that depending on the local pH, a mammalian extracellular matrix-degrading enzyme, endo-beta-D glucuronidase (heparanase), which cleaves heparin sulfate proteoglycans, can function wither as an enzyme or as an adhesion molecule for CD4+ T lymphocytes. Herein, the involvement of heparanase in T cell-keratinocyte interactions was also probed. At 37 degree C and pH 7.2, radioactively labeled MIP-1 beta, RANTES, and heparanase bound to confluent layers of resting keratinocytes in a saturable and an heparan sulfate- or heparin-dependent manner, and thereby induced the adhesion of resting CD4+ T cells to keratinocytes. At a relatively acidic pH characteristic of inflammatory milieu, enzymatically active heparanase did not bind to the keratinocytes but, rather, inhibited the binding of MIP-1beta...Continue Reading

References

Jul 4, 1977·Advances in Experimental Medicine and Biology·I A Silver
Mar 1, 1991·The Journal of Clinical Investigation·H Lortat-JacobJ A Grimaud
Jun 1, 1991·The Journal of Cell Biology·C Nathan, M Sporn
Apr 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P D Yurchenco, J C Schittny
Oct 1, 1994·Current Opinion in Cell Biology·J Gailit, R A Clark
May 1, 1994·International Journal of Dermatology·G S WoodR A Warnke
Mar 1, 1994·Immunology Today·M Baggiolini, C A Dahinden
Aug 1, 1993·The Journal of Clinical Investigation·J C AnselC E Hart
Sep 1, 1993·The Journal of Clinical Investigation·H LarjavaJ Heino
Feb 1, 1993·Immunology Today·J D Bos, M L Kapsenberg

❮ Previous
Next ❯

Citations

May 8, 2001·European Journal of Gastroenterology & Hepatology·N P MichellM J Langman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.