Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure

Mechanisms of Ageing and Development
Arianna RomaniGiuseppe Valacchi

Abstract

Epidemiological evidences have correlated airbone particulate matter (PM) to adverse health effects, mainly linking to pulmonary and cardiovascular disease. Nevertheless, only recently, some studies reported detrimental effects of PM on other organs such as skin. In a recent work, we have reported increased oxidative and inflammatory responses in Reconstituted Human Epidermis (RHE) exposed to ambient particles (CAPs) and we also demonstrated the ability of CAPs to penetrate the skin tissue. The present study was aimed to better understand the cellular mechanisms beyond the oxidative changes induced by CAPs (5-10-25μg/mL) in human immortalized keratinocytes (HaCaT). After 24h of treatment, CAPs were able to enter the cells leading to a decrease in viability, increased levels of 4-hydroxinonenal products (4-HNE) and IL-1α release. Overall these data, suggest lipid and protein oxidative damage, as well as an increase of inflammatory response after being challenged with CAPs. In addition, 3h after CAPs exposure we found a significant increase in NF-kB and Nrf2 translocation into the nucleus. In contrast, no differences in gene expression and enzymatic activity of Nrf2 target genes were detected. This last finding could be explained...Continue Reading

Citations

Mar 22, 2019·Journal of the European Academy of Dermatology and Venereology : JEADV·E AraviiskaiaB Dreno
Jul 6, 2019·Biomolecules & Therapeutics·Ao Xuan ZhenJin Won Hyun
Aug 11, 2020·Critical Reviews in Food Science and Nutrition·David Fonseca HernandezLuis Mojica
Jan 24, 2019·Marine Drugs·Yu Jae HyunJin Won Hyun
May 16, 2019·BioFactors·Alessandra PecorelliGiuseppe Valacchi
May 24, 2020·Antioxidants & Redox Signaling·Giuseppe ValacchiPablo Evelson
Jun 7, 2020·Environmental Science and Pollution Research International·Franco CervellatiGiuseppe Valacchi
Jul 14, 2020·International Journal of Cosmetic Science·John C BiermanJohn E Oblong
Jul 28, 2020·Particle and Fibre Toxicology·Irini M DijkhoffBarbara Rothen-Rutishauser
May 8, 2020·Oxidative Medicine and Cellular Longevity·Elena FrigatoGiuseppe Valacchi
Oct 28, 2020·Toxicology and Industrial Health·Seunghyun LeeWanhyung Lee
Mar 28, 2021·The Science of the Total Environment·Ayesha AslamMohammed Zwawi
Jul 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ping DiaoLi Li

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