PMID: 11310791Apr 20, 2001Paper

Keratinocytes suppress TRP-1 expression and reduce cell number of co-cultured melanocytes - implications for grafting of patients with vitiligo

Pigment Cell Research
J PhillipsS Mac Neil

Abstract

A pilot study for grafting of patients with vitiligo using cultured epithelial autografts containing melanocytes gave disappointing clinical results, with pigmentation achieved in only one out of five patients. Irrespective of the fate of melanocytes grafted back onto the patients, we experienced problems in identifying melanocytes within these well-integrated keratinocyte sheets. This led us to explore the fate of these cells within these sheets in vitro and to seek to improve their number and function within the sheets. We report that the introduction of a fibroblast feeder layer can improve melanocyte number within melanocyte/keratinocyte co-cultures initially, but at very high keratinocyte density, there is a marked loss of melanocytes (as detected by staining for S100). Additionally, we found that keratinocytes not only down-regulate melanocyte number, but also pigmentary function; thus, it was possible to identify melanocytes that were S100 positive but tyrosinase-related protein-1 (TRP-1) negative in confluent well-integrated keratinocyte sheets. In summary, our data suggest that keratinocytes at high density initially suppress melanocyte pigmentation (as evidenced by a lack of TRP-1 expression) and then cause a physical...Continue Reading

Citations

Mar 7, 2003·Plastic and Reconstructive Surgery·Cengiz AcikelBahattin Celikoz
Dec 2, 2008·Pigment Cell & Melanoma Research·Rafael Falabella, Maria I Barona
May 17, 2006·International Journal of Dermatology·Virendra N Sehgal, Govind Srivastava
Jun 28, 2005·Biomaterials·Paula Clare EvesSheila Mac Neil
Sep 24, 2004·Physiological Reviews·Andrzej SlominskiJacobo Wortsman
Jul 15, 2017·The Journal of Craniofacial Surgery·Ke-Jia WuWen-Yu Wu
Jun 11, 2002·Analytical Biochemistry·Tie Chi LeiVincent J Hearing

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