Keratocyte apoptosis and corneal antioxidant enzyme activities after refractive corneal surgery

Eye
K BilgihanB Hasanreisoğlu

Abstract

Refractive corneal surgery induces keratocyte apoptosis and generates reactive oxygen radicals (ROS) in the cornea. The purpose of the present study is to evaluate the correlation between keratocyte apoptosis and corneal antioxidant enzyme activities after different refractive surgical procedures in rabbits. Rabbits were divided into six groups. All groups were compared with the control group (Group 1), after epithelial scraping (Group 2), epithelial scrape and photorefractive keratectomy (PRK) (traditional PRK: Group 3), transepithelial PRK (Group 4), creation of a corneal flap with microkeratome (Group 5) and laser-assisted in situ keratomileusis (LASIK, Group 6). Terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick-end labelling assay (to detect DNA fragmentation in situ) and light microscopy were used to detect apoptosis in rabbit eyes. Glutathione peroxidase (Gpx) and superoxide dismutase (SOD) activities of the corneal tissues were measured with spectrophotometric methods. Corneal Gpx and SOD activities decreased significantly in all groups when compared with the control group (P<0.05) and groups 2, 3 and 6 showed a significantly higher amount of keratocyte apoptosis (P<0.05). Not only a negative corre...Continue Reading

References

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Citations

Dec 18, 2004·Cornea·Tae-im KimMichael S Kook
Jun 27, 2013·PloS One·Aleksandar StojanovicTor Paaske Utheim
Jun 1, 2013·Current Eye Research·Metin EkinciGokcen Gokce
Feb 11, 2016·Current Eye Research·Shengsheng WeiXiaolian Su
May 1, 2010·Ophthalmic Surgery, Lasers & Imaging : the Official Journal of the International Society for Imaging in the Eye·Anthony Liu, Edward E Manche
Dec 14, 2007·Seminars in Cell & Developmental Biology·Natalie LassenVasilis Vasiliou
Jul 16, 2003·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·A NietoJ A Sanchis-Gimeno
Jan 23, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella M Y CheungTrevor Sherwin
Dec 13, 2005·Journal of Cataract and Refractive Surgery·Sergio Zaccharia ScalinciRenato Alberto Meduri
Aug 15, 2014·Journal of Ophthalmology·Safak KorkmazAhmet Hondur
Feb 7, 2007·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·L ScorolliR A Meduri
Mar 18, 2019·Biochemistry. Biokhimii︠a︡·V E BaksheevaI I Senin
Sep 10, 2020·Experimental Eye Research·Rodrigo Carlos de Oliveira, Steven E Wilson

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis