Ketamine induces reactive oxygen species and enhances autophagy in SV-HUC-1 human uroepithelial cells

Journal of Cellular Physiology
Zhengfei ShanDongfu Liu

Abstract

This study was aimed at exploring the underlying mechanisms of ketamine in the SV-40 immortalized human ureteral epithelial (SV-HUC-1) cells. The viability and apoptosis of SV-HUC-1 cells treated with 0.01, 0.1, and 1 mM ketamine were respectively detected via cell counting kit-8 (CCK-8) assay and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining. Reactive oxygen species (ROS) level was measured through ROS probe staining. Apoptosis-related proteins (B-cell lymphoma 2 [Bcl-2] and Bax) and autophagy-associated proteins (light chain 3-I [LC3-I] and LC3-II) were determined by western blot or immunofluorescent assay. Additionally, transmission electron microscopy (TEM) was used to evaluate the formation of autophagosomes. After cotreatment of 3-methyladenine (3-MA) or N-acetyl-l-cysteine (NAC), the biological functions of SV-HUC-1 cells were analyzed to determine the association of ROS with cell viability and autophagy. CCK-8 assay and TUNEL staining indicated that ketamine effectively decreased the viability of SV-HUC-1 cells and accelerated apoptosis of SV-HUC-1 cells through regulating the expression level of IKBα (phospho), nuclear factor кB (P65), Bcl-2, and Bax protein...Continue Reading

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Citations

Jun 27, 2020·Cancer Biology & Therapy·Chunhua ZhouLulu Zhai
Mar 27, 2020·Neurourology and Urodynamics·Daniele CastellaniMarco Dellabella
Jun 4, 2019·Frontiers in Psychiatry·Nils C Gassen, Theo Rein
Feb 26, 2021·Neural Plasticity·Xianquan AnYang Jin
Mar 23, 2021·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Hanqing TangTianzi Li
Jun 3, 2021·International Journal of Molecular Sciences·Dong-Ru HoChih-Shou Chen
Jun 10, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiang XieHuan Chen
Feb 23, 2020·Future Microbiology·João Batista de Andrade NetoHélio Vitoriano Nobre Júnior

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