Ketamine inhibits LPS-induced calcium elevation and NF-kappa B activation in monocytes

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
J SunJ G Xu

Abstract

To investigate whether ketamine could inhibit lipopolysaccharide (LPS)-induced intracellular calcium elevation and NF-kappa B activation in monocytes. Isolated rat monocytes were challenged with 10 microg/ml LPS with or without the presence of various concentrations of ketamine (10, 100, 1000 microM). Intracellular calcium was monitored by laser confocal microscopy. NF-kappa B activity of the nuclear extracts of monocytes was analyzed by electrophoretic mobility shift assay (EMSA). LPS provoked a significant calcium elevation and enhanced NF-kappa B activity in monocytes. Ketamine above concentration of 100 microM inhibited endotoxin-induced intracellular calcium elevation and NF-kappa B activity. Ketamine itself had no effect on either of them. These findings suggest that ketamine could suppress NF-kappa B in monocytes exposed to endotoxin, and this anti-inflammatory effect might act through attenuating intracellular calcium elevation.

Citations

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