PMID: 9523822Apr 2, 1998Paper

Ketamine inhibits monoamine transporters expressed in human embryonic kidney 293 cells

Anesthesiology
M NishimuraM Tohyama

Abstract

Ketamine has been characterized as having psychotomimetic and sympathomimetic effects. These symptoms have raised the possibility that ketamine affects monoaminergic neurotransmission. To elucidate the relation between ketamine and monoamine transporters, the authors constructed three cell lines that stably express the norepinephrine, dopamine, and serotonin transporters and investigated the effects of ketamine on these transporters. Human embryonic kidney cells were transfected using the Chen-Okayama method with the human norepinephrine, rat dopamine, and rat serotonin transporter cDNA subcloned into the eukaryotic expression vector. Using cells stably expressing these transporters, the authors investigated the effects of ketamine on the uptake of these compounds and compared them with those of pentobarbital. Inhibition analysis showed that ketamine significantly inhibited the uptake of all three monoamine transporters in a dose-dependent manner. The Ki (inhibition constant) values of ketamine on the norepinephrine, dopamine, and serotonin transporters were 66.8 microM, 62.9 microM, and 162 microM, respectively. Pentobarbital, a typical general anesthetic agent with no psychotic symptoms, did not affect the uptake of monoamine...Continue Reading

References

Oct 25, 1991·Science·B J HoffmanM J Brownstein
Oct 1, 1990·Trends in Pharmacological Sciences·J WillettsJ D Leander
Sep 23, 1988·European Journal of Pharmacology·S W Tam, A Z Zhang
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama

❮ Previous
Next ❯

Citations

Oct 16, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Bong-Ho KoKyung-Han Lee
Mar 18, 2000·European Journal of Pharmacology·P B DePetrilloJ Dee Higley
Dec 20, 1999·Neuroscience Letters·M Nishimura, K Sato
Jul 25, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Shigeyuki YamamotoEdward F Domino
Apr 12, 2005·Journal of Cardiovascular Pharmacology·Allyson J Bennett, Paolo B DePetrillo
Mar 15, 2006·CNS Drugs·Kim Wolff, Adam R Winstock
Jun 20, 2014·Anesthesia and Analgesia·Jana Sawynok
Jan 31, 2007·European Journal of Anaesthesiology·E GasconL Vutskits
Jan 3, 2016·Progress in Neurobiology·Rodrigo Machado-VieiraCarlos A Zarate
Apr 4, 2013·Journal of Psychiatric Research·Heidi Kaastrup MüllerBetina Elfving
Sep 26, 2008·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Yejun Zhao, Lena Sun
Feb 13, 2007·Neurotoxicology and Teratology·L K M WrightM G Paule
Dec 7, 2006·Neurochemistry International·Miklos G VeghGyörgy Lévay
Mar 31, 2016·Clinical Pharmacokinetics·Marko A PeltoniemiTeijo I Saari
Jun 4, 2011·Medical Hypotheses·Zhiqiang ZhouChun Yang
Mar 31, 2015·Biochemical Pharmacology·Elena DaleConnie Sánchez
Jun 6, 2016·Progress in Neuro-psychopharmacology & Biological Psychiatry·Kristian Gaarn du JardinConnie Sanchez
Jun 23, 2016·Depression and Anxiety·Sean Sassano-HigginsMark Gold
Jul 30, 2016·The Journal of Pharmacology and Experimental Therapeutics·Adem CanTodd D Gould
May 19, 2017·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Ahmad M Abd El-RahmanMohamed A M Mostafa
Nov 4, 2017·The International Journal of Neuropsychopharmacology·Thomas LiebeMartin Walter
Apr 4, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Laszlo VutskitsJozsef Z Kiss
Feb 23, 2007·Journal of Neurophysiology·A Kepecs, S Raghavachari
Jun 28, 2018·Pharmacological Reviews·Panos ZanosTodd D Gould
May 7, 2020·Reviews in the Neurosciences·Rebecca McMillan, Suresh D Muthukumaraswamy
Sep 9, 2020·Anesthesiology·Jacob GitlinOluwaseun Akeju
Dec 15, 2016·Frontiers in Human Neuroscience·Linda Li, Phillip E Vlisides

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.